BOULA A. Yvette, Cameroon

Centre Mère et Enfant laboratory

Presenter Of 1 Presentation

DISTRIBUTION OF STREPTOCOCCUS PNEUMONIAE SEROTYPES AFTER THE INTRODUCTION OF PCV 13 IN CAMEROON (ID 266)

Abstract

Background

In July 2011, the 13- valent pneumococcal conjugate vaccine (PCV 13) was introduced into the routine immunization program in Cameroon.After the introduction of this vaccine, post-vaccine surveillance continued to monitor vaccine effectiveness and detect the emergence of new strains

We describe the pneumococcal serotypes that circulated after the introduction of PCV 13.

Methods

Retrospective review data from sentinel laboratory-based surveillance system from 2011 to 2018. Lumbar puncture was performed on children under five years admitted with suspected meningitis at the paediatric hospital in Yaoundé. Cerebrospinal fluid (CSF) specimens were tested by Gram stain, culture, latex agglutination and/ or Polymerase Chain Reaction (PCR). Serotyping/grouping, and/or whole genome sequencing were performed on Streptococcus pneumoniae isolates

Results

Among 8108 CSF specimens tested, 116 cases of meningitis were confirmed .89(76.7%) were positive for Streptococcus pneumoniae. PCR serotyping identified 19(39.5%) and 29(60.4%) S. pneumoniae PCV13 and non-PCV13 serotypes respectively. The most frequent non vaccine pneumococcal serotype was serotype 2 (13.7%) and the most frequent vaccine pneumococcal serotypes were 6A/ 6B (26%)

Conclusions

PCV 13 serotypes still remain in circulation . There is need for continuous surveillance and monitoring how vaccines can affect the evolution of strains over a long period of time

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Author Of 4 Presentations

PREVALENCE OF PNEUMOCOCCUS SEROTYPES IN MENINGITIS AT THE MOTHER AND CHILD CENTRE AFTER INTRODUCTION OF PCV13 VACCINE IN CAMEROON BETWEEN 2013 TO 2018. (ID 33)

Abstract

Background

The major cause of morbidity and mortality in children below 5 years old, Streptococcus pneumoniae meningitis is a global scourge. Since 1 July 2011, the PCV13 pneumococcal vaccine has been introduced into the routine immunization program in Cameroon. We describe the serotypes that caused pneumococcal meningitis after introducing the vaccine.

Methods

Pneumococcal isolates of children below 5 years of age between January 2013 and December 2018 sentinel surveillance for meningitis conducted at the Mother and Child Center, were serotyped and sequentially multiplexed polymerase chain reaction.

Results

Of the 64 cases of confirmed streptococcal pneumoniae meningitis, 41 (64.06%) were analyzed for serotype identification and 34 serotypes were obtained. The most common are: 6A-6B-15B-2-4-5-12F-7A-7F-12A-12B-16F-17F-25F-44. The highest frequency of types 6A and 6B (14.71%) is noted. We observe a high frequency of serotypes 2 and 5 in the age group 0-5Month: 3/16 (18.75%). 2/14 (14.28%) of children in the age group 6-23Month have serotype 12A / 12B / 12F / 44/4. As for the age group of 24-59M, the most represented serotype is 15B: 2/11(18.18%).

Conclusions

Although the PCV13 vaccine is beneficial in Cameroon, it is necessary to constantly monitor the emergence of non-vaccinal serotypes because from the results, we note the emergence of other serotypes.

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DISTRIBUTION OF STREPTOCOCCUS PNEUMONIAE SEROTYPES AFTER THE INTRODUCTION OF PCV 13 IN CAMEROON (ID 266)

Abstract

Background

In July 2011, the 13- valent pneumococcal conjugate vaccine (PCV 13) was introduced into the routine immunization program in Cameroon.After the introduction of this vaccine, post-vaccine surveillance continued to monitor vaccine effectiveness and detect the emergence of new strains

We describe the pneumococcal serotypes that circulated after the introduction of PCV 13.

Methods

Retrospective review data from sentinel laboratory-based surveillance system from 2011 to 2018. Lumbar puncture was performed on children under five years admitted with suspected meningitis at the paediatric hospital in Yaoundé. Cerebrospinal fluid (CSF) specimens were tested by Gram stain, culture, latex agglutination and/ or Polymerase Chain Reaction (PCR). Serotyping/grouping, and/or whole genome sequencing were performed on Streptococcus pneumoniae isolates

Results

Among 8108 CSF specimens tested, 116 cases of meningitis were confirmed .89(76.7%) were positive for Streptococcus pneumoniae. PCR serotyping identified 19(39.5%) and 29(60.4%) S. pneumoniae PCV13 and non-PCV13 serotypes respectively. The most frequent non vaccine pneumococcal serotype was serotype 2 (13.7%) and the most frequent vaccine pneumococcal serotypes were 6A/ 6B (26%)

Conclusions

PCV 13 serotypes still remain in circulation . There is need for continuous surveillance and monitoring how vaccines can affect the evolution of strains over a long period of time

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CHANGES IN ANTIMICROBIAL SUSCEPTIBILITY OF STREPTOCOCCUS PNEUMONIAE AMONG CHILDREN UNDER-FIVE YEARS ADMITTED WITH BACTERIAL MENINGITIS IN YAOUNDÉ AFTER PNEUMOCOCCAL CONJUGATE VACCINE INTRODUCTION IN CAMEROON. (ID 291)