José de Jesús Olivares-Trejo, Mexico
Universidad Autónoma de la Ciudad de México Posgrado en Ciencias GenómicasPoster Author Of 1 e-Poster
FINDING A SURFACE BIORECEPTOR FOR IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE.
Presenter of 1 Presentation
FINDING A SURFACE BIORECEPTOR FOR IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE. (ID 1081)
Abstract
Background
Streptococcus pneumoniae is the principal etiological agent of otitis and meningitis in children around the world. In the last years a strain of this pathogen resistant to optochin has been detected. Therefore, there is a possibility to find false negatives in the current methods used to identify this pathogen. Then it is necessary to develop new identification methods.
Methods
Here, a strategy is presented to select a molecule that binds to S. pneumoniae surface, which would allow the specific identification of this pathogen. This strategy consists on using short single-stranded DNA (aptamer).
Results
The selected aptamer was able to bind the S. pneumoniae surface, but not to other bacteria such as: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus pyogenes and Pseudomonas aeruginosa, with which can be confused during the identification processes. The aptamer selected was cloned in a plasmid vector and sequenced to know the sequence and to model the 3D structure. The aptamer was coupled to gold nanoparticles to obtain a bio-conjugated molecule and this structure also bound the S. pneumoniae surface indicating that the structure was not altered by the nanoparticles.
Conclusions
This aptamer is proposed as a bioreceptor to design a biosensor for the easy, fast and specific identification of S. pneumoniae.
Author Of 1 Presentation
FINDING A SURFACE BIORECEPTOR FOR IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE. (ID 1081)
Abstract
Background
Streptococcus pneumoniae is the principal etiological agent of otitis and meningitis in children around the world. In the last years a strain of this pathogen resistant to optochin has been detected. Therefore, there is a possibility to find false negatives in the current methods used to identify this pathogen. Then it is necessary to develop new identification methods.
Methods
Here, a strategy is presented to select a molecule that binds to S. pneumoniae surface, which would allow the specific identification of this pathogen. This strategy consists on using short single-stranded DNA (aptamer).
Results
The selected aptamer was able to bind the S. pneumoniae surface, but not to other bacteria such as: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus pyogenes and Pseudomonas aeruginosa, with which can be confused during the identification processes. The aptamer selected was cloned in a plasmid vector and sequenced to know the sequence and to model the 3D structure. The aptamer was coupled to gold nanoparticles to obtain a bio-conjugated molecule and this structure also bound the S. pneumoniae surface indicating that the structure was not altered by the nanoparticles.
Conclusions
This aptamer is proposed as a bioreceptor to design a biosensor for the easy, fast and specific identification of S. pneumoniae.