AKUNNA P. NWIGWE, Nigeria

Poster Author Of 1 e-Poster

Online Abstracts Vaccines - Pneumococcal Vaccines Development C1 Pneumococcal Vaccines Development

EVALUATION OF THE IMMUNOSTIMULATORY EFFECT OF PLEUROTUS OSTREATUS AGAINST STREPTOCOCCUS PNEUMONIAE INFECTIONS

Presentation Type
Abstract_Submission
Presentation Topic
C1 Pneumococcal Vaccines Development

Presenter Of 1 Presentation

EVALUATION OF THE IMMUNOSTIMULATORY EFFECT OF PLEUROTUS OSTREATUS AGAINST STREPTOCOCCUS PNEUMONIAE INFECTIONS (ID 27)

Session Name
Vaccines - Pneumococcal Vaccines Development

Abstract

Background

Mushrooms are white rot fungi regarded as one of the well-known food possessing various kinds of biopharmaceuticals compounds. Pleurotus ostreatus is an edible mushroom with history of medicinal uses.

Methods

Phytochemical constituents of the methanol extract were quantified while pure isolate of Streptococcus pneumoniae was obtained using standard protocols. The effect of the extract on cellular immune responses was assessed by Phagocytosis Evaluation and Nitroblue Tetrazolium Tests. In vivo study against Streptococcal infection was evaluated using the neutrophil adhesion, carbon clearance and Hemagglutination titre tests in mice.

Results

Methanol extracts of Pleurotus revealed the presence of flavonoids (6.41%), alkaloids (10.01%), saponins (2.02%), phenols (1.55%), carbohydrate (14.05%), and proteins (45.09%). The percentage stimulation of the polymorphonuclear neutrophils (PMNs) was obtained as 53% for (500mg/ml), while the Nitroblue Tetrazolium test score ranged from 58.00±9.64 for 500mg/ml to 27.33±4.84 for 125mg/ml. Significant number of PMNs adhered to nylon fibre while antibody titre and phagocytic index increased in concentration dependent manner.

Conclusions

Pleurotus ostreatus has high carbohydrate and protein contents. It showed a significant stimulation of the cell mediated and humoral immunity against Streptococcus pneumoniae infections.

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Author Of 1 Presentation

EVALUATION OF THE IMMUNOSTIMULATORY EFFECT OF PLEUROTUS OSTREATUS AGAINST STREPTOCOCCUS PNEUMONIAE INFECTIONS (ID 27)

Session Name
Vaccines - Pneumococcal Vaccines Development

Abstract

Background

Mushrooms are white rot fungi regarded as one of the well-known food possessing various kinds of biopharmaceuticals compounds. Pleurotus ostreatus is an edible mushroom with history of medicinal uses.

Methods

Phytochemical constituents of the methanol extract were quantified while pure isolate of Streptococcus pneumoniae was obtained using standard protocols. The effect of the extract on cellular immune responses was assessed by Phagocytosis Evaluation and Nitroblue Tetrazolium Tests. In vivo study against Streptococcal infection was evaluated using the neutrophil adhesion, carbon clearance and Hemagglutination titre tests in mice.

Results

Methanol extracts of Pleurotus revealed the presence of flavonoids (6.41%), alkaloids (10.01%), saponins (2.02%), phenols (1.55%), carbohydrate (14.05%), and proteins (45.09%). The percentage stimulation of the polymorphonuclear neutrophils (PMNs) was obtained as 53% for (500mg/ml), while the Nitroblue Tetrazolium test score ranged from 58.00±9.64 for 500mg/ml to 27.33±4.84 for 125mg/ml. Significant number of PMNs adhered to nylon fibre while antibody titre and phagocytic index increased in concentration dependent manner.

Conclusions

Pleurotus ostreatus has high carbohydrate and protein contents. It showed a significant stimulation of the cell mediated and humoral immunity against Streptococcus pneumoniae infections.

Hide