Fuminori Sakai, United States of America

Emory University Rollins School of Public Health Global Health

Author Of 1 Presentation

PNEUMOTAC: A HIGH-THROUGHPUT PLATFORM FOR SEROTYPING STREPTOCOCCUS PNEUMONIAE (ID 242)

Abstract

Background

Sensitive, high-throughput platforms, for pneumococcal serotyping are essential for vaccine effectiveness studies. We developed PneumoTAC, a TaqMan array card (TAC) platform for the rapid identification of most S. pneumoniae serotypes.

Methods

PneumoTAC reactions underwent a thorough analytical validation including studies of limit of detection (LOD) and sensitivity. The performance of PneumoTAC was investigated on blinded pneumococcal isolates (N=131), and in N=84 clinical specimens from pneumococcal disease episodes [cerebrospinal fluid samples (CSF, N=71), and blood cultures (N=13)].

Results

The LOD of assays in PneumoTAC spanned between 2 and 21.4 genome equivalents. A sensitivity of 100%, and 98.8%, was achieved for S. pneumoniae species confirmation using DNA from isolates, and clinical specimens, respectively. The sensitivity for the detection of the serotypes/serogroups was 94% using DNA from isolates. In clinical specimens PneumoTAC detected a serotype in N=80 (96.3%) of samples, whereas a multiplex (m)PCR approach detected a serotype in N=55 (66%) of specimens. The correlation between the serotype obtained by mPCR and that obtained by PneumoTAC was 96.3% (N=53).

Conclusions

We developed PneumoTAC a multi-target platform with increased serotype coverage, compared to other TAC assays and multiplex PCR approaches, to identify 94 S. pneumoniae serotypes/serogroups in a single run.

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