Elizabeth Miller,
Poster Author Of 1 e-Poster
PHYLOGENETIC INFERENCE OF THE TRANSMISSION DIRECTION OF PNEUMOCOCCAL INFECTION, A VALIDATION STUDY
- Jada Hackman, United Kingdom
- Carmen L. Sheppard, United Kingdom
- Ben Sobkowiak, United Kingdom
- Jody Phelan, United Kingdom
- Sonal Shah, United Kingdom
- David Litt, United Kingdom
- Norman K. Fry, United Kingdom
- Martin Hibberd, United Kingdom
- Elizabeth Miller,
- Stefan Flasche, United Kingdom
- Stéphane Hué, United Kingdom
Author Of 2 Presentations
PHYLOGENETIC INFERENCE OF THE TRANSMISSION DIRECTION OF PNEUMOCOCCAL INFECTION, A VALIDATION STUDY (ID 719)
- Jada Hackman, United Kingdom
- Carmen L. Sheppard, United Kingdom
- Ben Sobkowiak, United Kingdom
- Jody Phelan, United Kingdom
- Sonal Shah, United Kingdom
- David Litt, United Kingdom
- Norman K. Fry, United Kingdom
- Martin Hibberd, United Kingdom
- Elizabeth Miller,
- Stefan Flasche, United Kingdom
- Stéphane Hué, United Kingdom
Abstract
Background
Sustaining herd protection via reduced dose schedules may ameliorate pneumococcal conjugate vaccine costs. However, there is limited understanding of pneumococcal transmission pathways and their role in herd immunity. We aimed to develop and validate phylogenetic methods for detecting the occurrence and direction of pneumococcal transmission.
Methods
Based on the timing of serotype-specific carriage within a household, 10 likely transmission pairs and the corresponding transmission direction were identified from a longitudinal study of nasopharyngeal carriage in the UK and sequenced by whole genome sequencing. Any metadata were blinded, and linkage and the transmission direction inferred from the genomic data alone using Phyloscanner.
Results
Unblinding revealed that transmission pair linkage via genomics was identical to that based on epidemiological criteria. One instance of co-colonization was detected and only the dominant serotype was transmitted. All transmission pairs had moderate to strong phylogenetic signals suggesting transmission direction, however, only 6/10 directions were concordant with the epidemiological metadata.
Conclusions
Phylogenetics did successfully predict transmission pairs in this small sample. To improve the inference of transmission direction we will consider factors including sequencing coverage, degrees of intra-host diversity, and phylogenetic uncertainty, although concordance with epidemiolocal metadata may be limited by imperfect sensitivity of culture-based tests for pneumococcal detection.
PNEUMOCOCCAL CARRIAGE AND ANTIBODY PERSISTENCE FOLLOWING PCV13 DELIVERED AS ONE PRIMARY AND ONE BOOSTER (1+1) VERSUS TWO PRIMARY DOSES AND A BOOSTER IN UK INFANTS (ID 900)
- David Goldblatt, United Kingdom
- Nick Andrews, United Kingdom
- Carmen L. Sheppard, United Kingdom
- Samuel Rose,
- Parvinder Aley,
- Lucy Roalfe, United Kingdom
- Hannah Robinson,
- Emma Pearce,
- Emma Plested,
- Marina Johnson,
- David Litt, United Kingdom
- Norman K. Fry, United Kingdom
- Matthew Snape, United Kingdom
- Elizabeth Miller,