E-POSTER GALLERY (ID 409)

P-0112 - Induction of mitochondrial-mediated apoptosis by methanol extract of Cajanus cajan

Abstract Control Number
1537
Abstract Body
Induction of mitochondrial-mediated apoptosis by methanol extract of Cajanus cajan
Alao O Oa,b and Olorunsogo O.Oa aDepartment of Biochemistry, University of Ibadan, Nigeria bDepartment of Biochemistry, Lead City University, Ibadan, Nigeria. *All correspondence: olajualao@yahoo.co.uk +2348034728112Background: Attention has recently focused on mitochondrial-mediated apoptosis as a signaling pathway that is disrupted during carcinogenesis. The chemopreventive effects of dietary phytochemicals can be explored in order to combat conditions emanating from dysregulation of mitochondria-mediated apoptosis. Cajanus cajan is a medicinal plant commonly used in Nigeria for the traditional treatment of many diseases such as jaundice, measles dysentery and tumours. This study aims to investigate the effects of Cajanus cajan on the opening of the mitochondrial membrane permeability transition(MMPT) pore, ATPase activities, liver enzyme activities and immunohistochemical expression of apoptotic biomarkers in rat liver. Methods: Eighteen male Wistar rats (150±2.00 g) were assigned into three groups of six animals each and orally administered corn oil (control), 100 and 200 mg/kg MECC once daily for thirty consecutive days. Mitochondrial membrane permeability transition and ATPase activity were determined spectrophotometrically. Caspase 3, caspase 9 and cytochrome C release were determined by immunohistochemistry.Results: In vitro, at 10 and 30 µg/mL pore opening was not observed , however at 50 and 70 µg/mL MECC induced pore opening by 7 and 13 folds. Similarly, enhancement of ATPase activities was also observed. In the in vivo studies; the MECC induced opening at 200 mg/kg bwt.There was a significant increase in the activities of AST, ALT and ALP and caspase 3, caspase 9 and cytochrome C were activated Conclusion: Methanol extract of Cajanus cajan induced mitochondrial -mediated apoptosis in normal rat liver . Hence Cajanus cajan can be used in the management of conditions associated with dysregulation of apoptosis.
Keywords: Mitochondria membrane permeability transition pore : apoptosis and Cajanus cajan