Anna R. Giuliano (United States of America)

Moffitt Cancer Center Center for Immunization and Infection Research in Cancer

Presenter of 5 Presentations

 Conference Calendar
Public Health / Epidemiology / Other Public Health/Epidemiology Research ePoster

ORAL HPV PREVALENCE ASSESSMENT BY LINEAR ARRAY VS. SPF10 PCR-DEIA-LIPA25 SYSTEM IN THE HUMAN PAPILLOMAVIRUS INFECTION IN MEN (HIM) STUDY (ID 910)

Session Date
07/21/2020
Session Time
10:00 - 17:00
Room
ePoster
Session Type
Poster Viewing - 20-24 July
Session Name
Public Health / Epidemiology / Other Public Health/Epidemiology Research
Lecture Time
10:10 - 10:11
Presenter
Authors

Abstract

Introduction

Oral human papillomavirus (HPV) attributable oropharyngeal cancers are on the rise in many countries. Studies of oral HPV natural history are needed to fill gaps in knowledge. In studies to date, oral HPV infections are commonly detected using oral gargle samples. However, the optimal method for HPV genotyping oral gargle specimens has not been previously evaluated.

Methods

Oral gargle samples from 1455 HPV Infection in Men (HIM) study participants were HPV genotyped using two different methods: Linear Array and the SPF10 PCR-DEIA-LiPA25. The sensitivity of the two tests for detecting individual HPV types and grouped HPV types, high-risk HPV, low-risk HPV, grouped 4-HPV-vaccine types, and grouped 9-HPV-vaccine-types, and the degree of concordance between the two tests were assessed. We also examined whether socio-demograhpic and behavioral factors were associated with concordance between the two assays.

Results

The sensitivity of SPF10 PCR-DEIA-LiPA25 was higher than Linear Array, with the exception of HPV 70, for the detection of oral HPV. The prevalence ratio of SPF10 PCR-DEIA-LiPA25 to Linear Array varied between 1 and 9 for individual HPV genotypes, excluding HPV 70, and between 3.8 and 4.4 for grouped 4-valent and 9-valent HPV vaccine types, respectively. There was no association between socio-demographic and behavioral factors and discordance in results between the two tests for oral HPV 16 detection.

Conclusions

SPF10 PCR-DEIA-LiPA25 was more sensitive than Linear Array for detecting HPV in oral gargle samples. Given the growing importance of detecting oral HPV infection for oral HPV natural history studies and vaccine effectiveness evaluation, we recommend using methods with higher sensitivity such as SPF10 PCR-DEIA-LiPA25 for detecting HPV in oral gargle samples.

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Workshop 1: HPV Vaccine Hall B

Introduction by Chairs (ID 1713)

Session Date
07/20/2020
Session Time
09:00 - 10:05
Room
Hall B
Session Type
Public Health
Session Name
Workshop 1: HPV Vaccine
Lecture Time
09:00 - 09:03
Presenter
Authors
Workshop 2: Burden of Disease Hall A

Natural history of HPV among males: Implications for prevention and control (ID 48)

Session Date
07/20/2020
Session Time
11:00 - 12:25
Room
Hall A
Session Type
Interdisciplinary
Session Name
Workshop 2: Burden of Disease
Lecture Time
11:43 - 12:03
Presenter
Authors
Public Health / Epidemiology / Epidemiology: Natural History/Risk Factors ePoster

FACTORS ASSOCIATED WITH ANY, HIGH RISK, AND LOW RISK ORAL HUMAN PAPILLOMAVIRUS PREVALENCE: THE HPV INFECTION IN MEN (HIM) STUDY (ID 868)

Session Date
07/21/2020
Session Time
10:00 - 17:00
Room
ePoster
Session Type
Poster Viewing - 20-24 July
Session Name
Public Health / Epidemiology / Epidemiology: Natural History/Risk Factors
Lecture Time
10:13 - 10:14
Presenter
Authors

Abstract

Introduction

Given the rise of HPV-attributable oropharyngeal cancers across the globe, especially among men residing in high-income countries, it is important to understand the risk factors of oral HPV infections. We utilized a multinational sample of men residing in Brazil, Mexico and the US to examine factors associated with oral HPV prevalence separately for high-risk and low-risk HPV, and by country of residence.

Methods

DNA was extracted from adequate oral gargle samples collected from men enrolled in the HPV Infection in Men (HIM) study (N=3,127) and HPV genotyped using the SPF10 PCR-DEIA-LiPA25 system. Log-binomial multivariate models were fit separately for vaginal/anal sex and oral sex variables, after adjusting for age, country of residence, marital status, pack-years of cigarettes smoked, and history of bleeding gums.

Results

Reporting more than 19 lifetime number of sexual partners was significantly associated with any [aPR: 2.9 (1.8,4.6)], high-risk [aPR: 2.8 (1.6,4.8)], and low-risk HPV [aPR: 3.3 (1.3,8.2)] compared to reporting two or less partners. Reporting 25 or more oral sex partners in the past 6 months was significantly associated with any [aPR: 1.7 (1.2,2.5)] and high-risk [aPR: 1.7 (1.1,2.7)] HPV compared to reporting no oral sex partners. Similarly, reporting 3 or less number of days since last oral sex was significantly associated with any [aPR: 2.0 (1.1,3.5)] and high-risk [aPR: 2.2 (1.2,4.3)] HPV compared to reporting never having had oral sex during their lifetime.

Conclusions

Lifetime number of sexual partners was independently significantly associated with both high-risk and low-risk oral HPV prevalence while oral sex related variables were significantly associated with only high-risk HPV prevalence. These different risk-factor profiles of high-risk and low-risk oral HPV prevalence warrant further study.

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Public Health / Epidemiology / Epidemiology: Natural History/Risk Factors ePoster

GENITAL VS ANAL CANAL VS ORAL HPV PREVALENCE IN THE HPV INFECTION IN MEN (HIM) STUDY (ID 892)

Session Date
07/21/2020
Session Time
10:00 - 17:00
Room
ePoster
Session Type
Poster Viewing - 20-24 July
Session Name
Public Health / Epidemiology / Epidemiology: Natural History/Risk Factors
Lecture Time
10:14 - 10:15
Presenter
Authors

Abstract

Introduction

Human papillomavirus (HPV) is a known cause of anogenital cancers and a subset of oropharyngeal cancers among men across the globe. Little is known about simultaneous prevalence of HPV in genital, anal canal, and oral anatomic sites among men. In this study, we assessed the pairwise association of HPV infection in these three sites and the age distribution at each site.

Methods

Participants of the HPV Infection in Men (HIM) cohort study who had data for anal, genital, and oral HPV prevalence in the same study visit were included (N = 739). Genital and anal canal HPV was genotyped using Roche Linear Array whereas oral HPV was genotyped using the SPF10 PCR-DEIA-LiPA25 assay. Pairwise association of HPV infection between the three anatomic sites and their age distributions were assessed using log binomial models.

Results

Prevalence of any HPV infection in genital, anal canal, and oral sites was 41.8%, 25.7%, and 10.1% respectively, and high-risk HPV infection was 32.2%, 19.1%, and 7.4% respectively. Anal infection was significantly associated with genital infection for HPV types 16, 39, 45, 51, 56, 58, 59, 6, 11, 53, 54 and 66. Oral infection was significantly associated with genital infection for HPV types 51, 56, 59, and 66 whereas significant association with anal infection was observed for HPV types 35 and 6. Any and high-risk genital HPV prevalence generally decreased with increasing age whereas anal HPV prevalence first decreased with age but then increased among men aged 51-73 years. Oral HPV demonstrated unimodal distribution, with peak prevalence among men aged 31-40 years.

Conclusions

Occurrence of simultaneous anal and genital HPV infections was observed for more HPV genotypes than oral-genital or oral-anal sites. Different HPV types were co-detected at oral-anal sites compared to anal-genital and oral-genital sites. Age-pattern of HPV prevalence differed between the three sites.

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Moderator of 4 Sessions

 Conference Calendar
Public Health

00040 - Workshop 1: HPV Vaccine (ID 10)

Session Type
Public Health
Session Date
07/20/2020
Session Time
09:00 - 10:05
Room
Hall B
Chair(s)
Public Health Oral Session

00690 - Oral Session 5: VACCINATION II. Implementation, dissemination, and communication (ID 32)

Session Type
Public Health Oral Session
Session Date
07/22/2020
Session Time
08:45 - 10:10
Room
Hall F
Chair(s)

Session Webcast

Dedicated Symposium

00980 - Elimination (ID 56)

Session Type
Dedicated Symposium
Session Date
07/24/2020
Session Time
09:00 - 10:05
Room
Hall A
Chair(s)

Session Webcast

Clinical Science Oral Session

01020 - Oral Session 10: Vaccines Clinical Aspects (ID 83)

Session Type
Clinical Science Oral Session
Session Date
07/24/2020
Session Time
12:35 - 14:05
Room
Hall B
Chair(s)

Session Webcast