R. Tomaszewski (Katowice, PL)
GCZDPresenter Of 2 Presentations
P026 - Statistical Image Analysis and Escort Histograms in Characterization of Articular Cartilage Repair in a Skeleton Animal Model
Abstract
Purpose
Statistical image analysis of an ensemble of digital images of histological samples is performed as an auxiliary investigation a result of the recently proposed method of articular cartilage repair utilizing growth plate chondrocytes in a skeleton animal model.
Methods and Materials
A fixed–shift model of maximal likelihood estimates of image histograms applied for monochromatic (grayscale) images or their RGB components confirms the statistically significant effect of the previously proposed medical treatment. The type of staining used to prepare images of histological samples is related to the visibility of the effectiveness of medical treatment.
Results
Hellinger distance of escort distributions for maximal likelihood estimates of image histograms of medically treated and control samples is investigated to identify grayscale (or RGB) intensities responsible for statistically significant difference of the estimates. A difference of Shannon entropy quantifying informational content of the histograms allows one to identify staining and image colors which are most suitable to visualize cluster formation typical for articular cartilage repair processes.
Conclusion
We conclude that the presented analysis causes us to believe that statistical image comparison can serve as a valuable tool for an auxiliary investigation of not–to–large sets of medical images. We claim that using statistical image analysis not only supports standard ‘human– based’ methods of selection of image parameters (such as color) or histological samples (such as applied staining) to represent and visualize the effectiveness of medical treatment but also can lead to new results with direct medical application and value.
P027 - Changes in the Avascular Area of the Meniscus Using Mesenchymal Stem Cells and Growth Plate Chondrocytes in a Pig Model
Abstract
Purpose
The aim of the study was to evaluate autologous growth plate chondrocytes’ impact on the healing process of a damaged meniscus in the avascular zone based on a growing animal model.
Methods and Materials
The study group consisted of 10 pigs at about three months of age. From each animal, chondrocytes from the iliac growth plate and from concentrated bone marrow were taken. Knee joints were divided into right (R) and left (L). The medial meniscus of the R knee joint was treated with a hyaluronic acid based scaffold incubated with bone marrow cells from marrow aspirates (nCHON). The medial meniscus of the L knee joint was treated with a hyaluronic acid-based scaffold incubated with bone marrow cells from marrow aspirates supplemented with immature chondrocytes isolated from growth plates (wCHON). The meniscus was damaged in the avascular zone in both knee joints. Followingly, the damaged part of the meniscus was filled with a scaffold with cells from the concentrated bone marrow and from growth plate chondrocytes. In the control group, a scaffold with concentrated bone marrow cells was used. After three months the animals were euthanized and preparations (microscopic slides) were made from the meniscus’ damaged part.
Results
A qualitative and quantitative analysis have been prepared. The wCHON group in comparison with the nCHON group showed a statistically significantly higher number of fusiform cells on the surface of the graft as well as better healing of the graft. In addition, the degree of vascularisation was higher in specimens from the wCHON group than in the nCHON group.
Conclusion
The results of our research on immature pig knees revealed that mesenchymal stem cell and growth plate chondrocytes could be treated as the cell source for meniscus reconstruction, and growth plate chondrocytes enhance healing processes in the avascular zone of the injured meniscus.
Meeting Participant Of
- R. Tomaszewski (Katowice, PL)
- T. Marin Fermin (Caracas, VE)
- Q. Liu (Beijing, CN)
- A. Botto-van Bemden (Holiday, US)
- R. Martin (Lausanne, CH)
- J. Carey (Philadelphia, US)
- M. Snow (Bromsgrove, GB)
- G. Gracitelli (Valinhos, BR)
- M. Karperien (Enschede, NL)
- L. Goodrich (Fort Collins, US)
- P. Emans (Maastricht, NL)
Presenter Of 2 Presentations
P026 - Statistical Image Analysis and Escort Histograms in Characterization of Articular Cartilage Repair in a Skeleton Animal Model
Abstract
Purpose
Statistical image analysis of an ensemble of digital images of histological samples is performed as an auxiliary investigation a result of the recently proposed method of articular cartilage repair utilizing growth plate chondrocytes in a skeleton animal model.
Methods and Materials
A fixed–shift model of maximal likelihood estimates of image histograms applied for monochromatic (grayscale) images or their RGB components confirms the statistically significant effect of the previously proposed medical treatment. The type of staining used to prepare images of histological samples is related to the visibility of the effectiveness of medical treatment.
Results
Hellinger distance of escort distributions for maximal likelihood estimates of image histograms of medically treated and control samples is investigated to identify grayscale (or RGB) intensities responsible for statistically significant difference of the estimates. A difference of Shannon entropy quantifying informational content of the histograms allows one to identify staining and image colors which are most suitable to visualize cluster formation typical for articular cartilage repair processes.
Conclusion
We conclude that the presented analysis causes us to believe that statistical image comparison can serve as a valuable tool for an auxiliary investigation of not–to–large sets of medical images. We claim that using statistical image analysis not only supports standard ‘human– based’ methods of selection of image parameters (such as color) or histological samples (such as applied staining) to represent and visualize the effectiveness of medical treatment but also can lead to new results with direct medical application and value.
P027 - Changes in the Avascular Area of the Meniscus Using Mesenchymal Stem Cells and Growth Plate Chondrocytes in a Pig Model
Abstract
Purpose
The aim of the study was to evaluate autologous growth plate chondrocytes’ impact on the healing process of a damaged meniscus in the avascular zone based on a growing animal model.
Methods and Materials
The study group consisted of 10 pigs at about three months of age. From each animal, chondrocytes from the iliac growth plate and from concentrated bone marrow were taken. Knee joints were divided into right (R) and left (L). The medial meniscus of the R knee joint was treated with a hyaluronic acid based scaffold incubated with bone marrow cells from marrow aspirates (nCHON). The medial meniscus of the L knee joint was treated with a hyaluronic acid-based scaffold incubated with bone marrow cells from marrow aspirates supplemented with immature chondrocytes isolated from growth plates (wCHON). The meniscus was damaged in the avascular zone in both knee joints. Followingly, the damaged part of the meniscus was filled with a scaffold with cells from the concentrated bone marrow and from growth plate chondrocytes. In the control group, a scaffold with concentrated bone marrow cells was used. After three months the animals were euthanized and preparations (microscopic slides) were made from the meniscus’ damaged part.
Results
A qualitative and quantitative analysis have been prepared. The wCHON group in comparison with the nCHON group showed a statistically significantly higher number of fusiform cells on the surface of the graft as well as better healing of the graft. In addition, the degree of vascularisation was higher in specimens from the wCHON group than in the nCHON group.
Conclusion
The results of our research on immature pig knees revealed that mesenchymal stem cell and growth plate chondrocytes could be treated as the cell source for meniscus reconstruction, and growth plate chondrocytes enhance healing processes in the avascular zone of the injured meniscus.