A. Pulatkan (İstanbul, TR)
Bezmialem Vakif University Orthopedics and TraumatologyPresenter Of 1 Presentation
P002 - Comparison of Three Cell-Free Matrix Scaffolds Used to Treat Osteochondral Lesions in a Rabbit Model
Abstract
Purpose
This study compared the macroscopic, histological, and scanning electron microscopy (SEM) characteristics of Chondro-Gide®, MaioRegen®, and poly-D,L-lactide-co-caprolactone (PLCL) cell-free scaffolds enhanced with small-diameter microfractures (SDMs) for osteochondral defects (OCDs) in a rabbit model.
Methods and Materials
In total, 54 knees from 27 rabbits were used in this study. Three rabbits were sacrificed at the beginning of the study to form an intact cartilage control group (group IC). An OCD model was created at the center of the trochlea and SDMs were generated in 24 rabbits. Rabbits with OCDs were divided into four groups (n=6 knees per group) according to the cell-free scaffold applied: Chondro-Gide® (group CG), MaioRegen® (group MA), PLCL (group PLCL), and a control group (group SDM). Half of the rabbits were sacrificed at 1 month after treatment, while the other half were sacrificed at 3 months after treatment. Healed cartilage was evaluated macroscopically (using International Cartilage Repair Society [ICRS] classification criteria) and histopathologically (using modified O’Driscoll scores and collagen staining). Additionally, cell-free scaffold morphologies were compared using SEM analysis.
Results
The histological characteristics of group IC samples were superior to those of all other groups, except group PLCL, at 3 months after treatment (p<0.05). In addition, the histological properties of group PLCL samples were superior to those of group SDM samples at both 1 and 3 months after treatment. Concerning type 2 collagen staining intensity, the groups were ranked from highest to lowest at 3 months after treatment, as follows: group PLCL (30.3±2.6)>group MA(26.6±1.2)>group CG(23.3±2.3)>group SDM(18.9±0.9). SEM analysis showed that the PLCL scaffold elasticity facilitated transmission of mechanical signals to adherent cells; scaffold pores also aided cell attachment and proliferation.
Conclusion
OCDs treated with enhanced SDM using cell-free PLCL scaffolds had superior histopathological and microenvironmental properties, more hyaline cartilage, and more type 2 collagen, compared with those treated using Chondro-Gide® or MaioRegen® scaffolds.
Presenter Of 1 Presentation
P002 - Comparison of Three Cell-Free Matrix Scaffolds Used to Treat Osteochondral Lesions in a Rabbit Model
Abstract
Purpose
This study compared the macroscopic, histological, and scanning electron microscopy (SEM) characteristics of Chondro-Gide®, MaioRegen®, and poly-D,L-lactide-co-caprolactone (PLCL) cell-free scaffolds enhanced with small-diameter microfractures (SDMs) for osteochondral defects (OCDs) in a rabbit model.
Methods and Materials
In total, 54 knees from 27 rabbits were used in this study. Three rabbits were sacrificed at the beginning of the study to form an intact cartilage control group (group IC). An OCD model was created at the center of the trochlea and SDMs were generated in 24 rabbits. Rabbits with OCDs were divided into four groups (n=6 knees per group) according to the cell-free scaffold applied: Chondro-Gide® (group CG), MaioRegen® (group MA), PLCL (group PLCL), and a control group (group SDM). Half of the rabbits were sacrificed at 1 month after treatment, while the other half were sacrificed at 3 months after treatment. Healed cartilage was evaluated macroscopically (using International Cartilage Repair Society [ICRS] classification criteria) and histopathologically (using modified O’Driscoll scores and collagen staining). Additionally, cell-free scaffold morphologies were compared using SEM analysis.
Results
The histological characteristics of group IC samples were superior to those of all other groups, except group PLCL, at 3 months after treatment (p<0.05). In addition, the histological properties of group PLCL samples were superior to those of group SDM samples at both 1 and 3 months after treatment. Concerning type 2 collagen staining intensity, the groups were ranked from highest to lowest at 3 months after treatment, as follows: group PLCL (30.3±2.6)>group MA(26.6±1.2)>group CG(23.3±2.3)>group SDM(18.9±0.9). SEM analysis showed that the PLCL scaffold elasticity facilitated transmission of mechanical signals to adherent cells; scaffold pores also aided cell attachment and proliferation.
Conclusion
OCDs treated with enhanced SDM using cell-free PLCL scaffolds had superior histopathological and microenvironmental properties, more hyaline cartilage, and more type 2 collagen, compared with those treated using Chondro-Gide® or MaioRegen® scaffolds.