M. Wrobel (Warszawa, PL)

Aleksander IV sp. z o.o. sp. komandytowa orthopaedics

Presenter Of 1 Presentation

Podium Presentation Stem Cells

12.2.9 - Assessment of Autologous BM Mesenchymal Stem Cells Use in ACL and Chondral Lesions. Preclinical Study on Animal Model

Presentation Topic
Stem Cells
Date
13.04.2022
Lecture Time
17:24 - 17:33
Room
Potsdam 3
Session Type
Free Papers
Disclosure
No Significant Commercial Relationship

Abstract

Purpose

We present a preclinical study of use of the Bone Marrow derived Mesenchymal Stem Cells in treatment of ACL tears and cartilage lesions on animal model.

Aims: To assess the technical aspects of the procedure of injection of the BM MSC’s suspended on the fibrin glue; to assess safety of MSC’s application for ACL and cartilage repair and the potential influence of MSC’s on ACL and cartilage healing by hstological analysis.

Presented paper is a part of EXPLORE ME project sponsored by National Center of Research and Development about the use of MSC’s in regenerative medicine.

Methods and Materials

Six animals (12 knees) were used. The animals were divided into the ACL (3) and cartilage (3) groups. In the ACL group iatrogenic damage of the ACL by its detachment at femoral insertion and reinsertion with Internal Brace technique during arthroscopic procedure were performed bilaterally. ACLs in all the right knees were injected with pure fibrin glue. ACLs in the left knees were injected with fibrin glue mixed with 5 x 106 expanded, autologous BM MSC’s.

In the cartilage group, iatrogenic damages of the cartilage at the weightbearing surface of the medial femoral condyle were created. The lesions were filled with hyaluronic scaffold injected with fibrin glue in right knees and with fibrin glue mixed with 5 x 106 expanded, autologous BM MSCs in the left knees in arthroscopic procedures bilaterally.

The animals were euthanised after three months follow up.

Results

Angiogenesis was observed in ACLs MSC+ comparing to control group. Angiogenesis was observed in cartilage samples from MSC-treated group, comparing to controls.

Conclusion

Injection of the MSC’s suspended in fibrin glue is safe and simple procedure. The cells stay inside treated structure. The finding of angiogenesis in treated cartilage wasn’t previously described in literature and requires further investigation.

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