D. Mainard (Nancy, FR)

Hôpital Central Service d'Orthopedie

Presenter Of 1 Presentation

Poster Biomaterials and Scaffolds

P045 - Elaboration of a Collagen-Enriched Bio-Ink for the Production of Zonal Specific Cartilage Substitutes

Presentation Topic
Biomaterials and Scaffolds
Date
13.04.2022
Lecture Time
09:30 - 09:30
Room
Exhibition Foyer
Session Name
7.3 - Poster Viewing / Coffee Break / Exhibition
Session Type
Poster Session
Disclosure
This work was supported by the “Fondation pour la Recherche Médicale”, grant number ECO201906008942 to Oceane MESSAOUDI.

Abstract

Purpose

In cartilage tissue engineering, 3D printing is thriving. The use of mesenchymal stem cells (MSCs), showing good proliferation and differentiation properties, enables the functionalization of printed constructs. The aim of our study was to assess the effect of different type I collagen quantity added in the bio-ink on the chondrogenic properties of MSCs to recreate the various layers of articular cartilage.

Methods and Materials

Bone marrow-MSCs were harvested from patients with osteoarthritis. After monolayer expansion, MSCs were suspended in an alginate-based bio-ink containing different quantity of soluble type I collagen: 0, 0.5, 1 and 5 mg/8mL of bio-ink. The constructs were bioextruded and polymerized. After 28 and 56 days of in vitro maturation, in a control media (ITS) or under a chondrogenic condition (ITS+TFG-β1), the cells viability, gene expressions (RT-qPCR) and matrix synthesis (histology and immunohistochemistry) were assessed.

Results

There was no effect of the global process on cell’s viability. After 28 days, the gene expressions show a chondrogenic differentiation of MSCs with TFG-β1. The quantity of 0.5mg enhanced the expression of chondrogenic genes, while 5mg induced a more osteogenic profile. At day 56, the matrix analyses highlight the positive effect of TFG-β1 on proteoglycan synthesis. An increase of type II collagen was observed with the lowest amount tested (0.5mg) whereas for the 5mg quantity was induced calcifications in the substitutes.

Conclusion

The use of an alginate-based bioink functionalized with MSCs, enables the production of cartilaginous substitutes with a chondrogenic gene expression profile and a proteoglycan rich matrix. The lowest quantity of collagen induces a more hyaline like matrix synthesis, suitable to recreate the more superficial cartilaginous layers. Furthermore, the quantity of 5mg causes the production of a calcified matrix more suitable for the calcified cartilage layer.

This work was supported by the “Fondation pour la Recherche Médicale”, grant number ECO201906008942 to Oceane MESSAOUDI.

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Presenter Of 1 Presentation

Biomaterials and Scaffolds

P045 - Elaboration of a Collagen-Enriched Bio-Ink for the Production of Zonal Specific Cartilage Substitutes

Abstract

Purpose

In cartilage tissue engineering, 3D printing is thriving. The use of mesenchymal stem cells (MSCs), showing good proliferation and differentiation properties, enables the functionalization of printed constructs. The aim of our study was to assess the effect of different type I collagen quantity added in the bio-ink on the chondrogenic properties of MSCs to recreate the various layers of articular cartilage.

Methods and Materials

Bone marrow-MSCs were harvested from patients with osteoarthritis. After monolayer expansion, MSCs were suspended in an alginate-based bio-ink containing different quantity of soluble type I collagen: 0, 0.5, 1 and 5 mg/8mL of bio-ink. The constructs were bioextruded and polymerized. After 28 and 56 days of in vitro maturation, in a control media (ITS) or under a chondrogenic condition (ITS+TFG-β1), the cells viability, gene expressions (RT-qPCR) and matrix synthesis (histology and immunohistochemistry) were assessed.

Results

There was no effect of the global process on cell’s viability. After 28 days, the gene expressions show a chondrogenic differentiation of MSCs with TFG-β1. The quantity of 0.5mg enhanced the expression of chondrogenic genes, while 5mg induced a more osteogenic profile. At day 56, the matrix analyses highlight the positive effect of TFG-β1 on proteoglycan synthesis. An increase of type II collagen was observed with the lowest amount tested (0.5mg) whereas for the 5mg quantity was induced calcifications in the substitutes.

Conclusion

The use of an alginate-based bioink functionalized with MSCs, enables the production of cartilaginous substitutes with a chondrogenic gene expression profile and a proteoglycan rich matrix. The lowest quantity of collagen induces a more hyaline like matrix synthesis, suitable to recreate the more superficial cartilaginous layers. Furthermore, the quantity of 5mg causes the production of a calcified matrix more suitable for the calcified cartilage layer.

This work was supported by the “Fondation pour la Recherche Médicale”, grant number ECO201906008942 to Oceane MESSAOUDI.

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