R. Vinestock (Rehovot, IL)
Weizmann Institute of science Molecular geneticsPresenter Of 1 Presentation
P030 - The Injured Enthesis Heals by a Novel Mechanism, Whereby ECM Secretion by Resident Cells Forms an Acellular Scar
Abstract
Purpose
Wound healing is a well-orchestrated process that restores the structure and function of an injured tissue through the involvement of the immune and vascular systems. Injuries to the enthesis, a transitional tissue that connects between tendon and bone, often result in scar formation and loss of mechanical properties, thereby severely affecting function and life quality. Nevertheless, a detailed investigation of the enthesis healing process has not been conducted.
Methods and Materials
To explore the healing process of the enthesis, we established a partial injury model in the Achilles enthesis of neonatal mice. Following an injury, sequential histological, molecular and behavioral assays were conducted, including in situ HCR, electron microscopy, gait analysis and qPCR.
Results
Shortly after injury, we observed an ECM plug within the lesion. The plug was composed of collagen type I and type II, secreted locally by resident enthesis cells, forming a permanent hypocellular scar. Classical healing attributes, such as the presence of inflammatory cells and angiogenesis, were not observed. Differential spatial distribution of markers of ER stress, autophagy and cell death was observed in the different zones of the enthesis. Eventually, gait capacities were restored, while no regeneration was observed.
Conclusion
These findings offer a novel healing mechanism, whereby local ECM secretion by resident cells forms an acellular plug in the absence of inflammation markers. This insight into the healing mechanism of a complex transitional tissue may promote new therapeutic strategies for enthesis injuries.
Presenter Of 1 Presentation
P030 - The Injured Enthesis Heals by a Novel Mechanism, Whereby ECM Secretion by Resident Cells Forms an Acellular Scar
Abstract
Purpose
Wound healing is a well-orchestrated process that restores the structure and function of an injured tissue through the involvement of the immune and vascular systems. Injuries to the enthesis, a transitional tissue that connects between tendon and bone, often result in scar formation and loss of mechanical properties, thereby severely affecting function and life quality. Nevertheless, a detailed investigation of the enthesis healing process has not been conducted.
Methods and Materials
To explore the healing process of the enthesis, we established a partial injury model in the Achilles enthesis of neonatal mice. Following an injury, sequential histological, molecular and behavioral assays were conducted, including in situ HCR, electron microscopy, gait analysis and qPCR.
Results
Shortly after injury, we observed an ECM plug within the lesion. The plug was composed of collagen type I and type II, secreted locally by resident enthesis cells, forming a permanent hypocellular scar. Classical healing attributes, such as the presence of inflammatory cells and angiogenesis, were not observed. Differential spatial distribution of markers of ER stress, autophagy and cell death was observed in the different zones of the enthesis. Eventually, gait capacities were restored, while no regeneration was observed.
Conclusion
These findings offer a novel healing mechanism, whereby local ECM secretion by resident cells forms an acellular plug in the absence of inflammation markers. This insight into the healing mechanism of a complex transitional tissue may promote new therapeutic strategies for enthesis injuries.