A. Changoor (Toronto, CA)
Lunenfeld Tanenbaum Research InstitutePresenter Of 1 Presentation
P170 - Independent Investigation of the Missouri Osteochondral Allograft Preservation System on Cartilage Quality
Abstract
Purpose
The Missouri Osteochondral Allograft Preservation System (MOPS) may extend the time when stored osteochondral tissues remain viable. This study aimed to provide an independent investigation of MOPS storage by evaluating chondrocyte viability, chondrocyte metabolism, and the cartilage extracellular matrix.
Methods and Materials
Femoral condyles from twelve female Arcott sheep (6 years, 70 ± 15 kg) were assigned to storage times of 0 (control), 14, 28, or 56 days. Sheep were assigned to standard of care [SOC, Lactated Ringer’s solution, cefazolin (1 g/L), bacitracin (50,000 U/L), 4°C storage] or MOPS [proprietary media, 22-25°C storage]. Samples underwent weekly media changes. Chondrocyte viability was assessed using Calcein AM/Ethidium Homodimer. Metabolism was evaluated with the Alamar blue assay and reported as Relative Fluorescent Units (RFU)/mg. Electromechanical properties were measured with the Arthro-BST, a device used to non-destructively compress cartilage and calculate a quantitative parameter (QP) that is inversely proportional to stiffness. Histological sections were stained with Safranin-O/Fast Green. A two-way ANOVA and Tukey’s post hoc were performed.
Results
MOPS maintained chondrocyte viability and viable cell density (VCD) longer than SOC-stored samples (Table 1). Cell metabolism in MOPS samples remained the same over the study duration but SOC was lower after 28 and 56 days compared to controls. Electromechanical QP measurements revealed no differences between storage methods at any time point. QP data could not be used to interpret changes over time because a mix of medial and lateral condyles were used and they have intrinsically different properties. Safranin-O/Fast Green showed proteoglycan diminished gradually beginning at the articular surface and progressing towards bone in SOC samples, while MOPS maintained proteoglycan over time (Figure 1).
Conclusion
MOPS exhibited superior viability, metabolic activity and proteoglycan retention compared to SOC, but did not maintain viability for 56 days. This work supports efforts to increase the supply of fresh osteochondral allografts for clinical use.
Presenter Of 1 Presentation
P170 - Independent Investigation of the Missouri Osteochondral Allograft Preservation System on Cartilage Quality
Abstract
Purpose
The Missouri Osteochondral Allograft Preservation System (MOPS) may extend the time when stored osteochondral tissues remain viable. This study aimed to provide an independent investigation of MOPS storage by evaluating chondrocyte viability, chondrocyte metabolism, and the cartilage extracellular matrix.
Methods and Materials
Femoral condyles from twelve female Arcott sheep (6 years, 70 ± 15 kg) were assigned to storage times of 0 (control), 14, 28, or 56 days. Sheep were assigned to standard of care [SOC, Lactated Ringer’s solution, cefazolin (1 g/L), bacitracin (50,000 U/L), 4°C storage] or MOPS [proprietary media, 22-25°C storage]. Samples underwent weekly media changes. Chondrocyte viability was assessed using Calcein AM/Ethidium Homodimer. Metabolism was evaluated with the Alamar blue assay and reported as Relative Fluorescent Units (RFU)/mg. Electromechanical properties were measured with the Arthro-BST, a device used to non-destructively compress cartilage and calculate a quantitative parameter (QP) that is inversely proportional to stiffness. Histological sections were stained with Safranin-O/Fast Green. A two-way ANOVA and Tukey’s post hoc were performed.
Results
MOPS maintained chondrocyte viability and viable cell density (VCD) longer than SOC-stored samples (Table 1). Cell metabolism in MOPS samples remained the same over the study duration but SOC was lower after 28 and 56 days compared to controls. Electromechanical QP measurements revealed no differences between storage methods at any time point. QP data could not be used to interpret changes over time because a mix of medial and lateral condyles were used and they have intrinsically different properties. Safranin-O/Fast Green showed proteoglycan diminished gradually beginning at the articular surface and progressing towards bone in SOC samples, while MOPS maintained proteoglycan over time (Figure 1).
Conclusion
MOPS exhibited superior viability, metabolic activity and proteoglycan retention compared to SOC, but did not maintain viability for 56 days. This work supports efforts to increase the supply of fresh osteochondral allografts for clinical use.