R. Baba (Sapporo, JP)
Hokkaido University Graduate School of Medicine Department of Orthopaedic SurgeryPresenter Of 1 Presentation
P172 - In Vitro Evaluation of the Effects of Ultra-purified Alginate on the Performance of Osteochondral Allografts Harvested from Pig Knees
Abstract
Purpose
Preventing degradation of osteochondral allografts (OCAs) before transplantation results in improvement of clinical outcomes after osteochondral allografting. Because ultra-purified alginate (UPAL) enhances cartilage repair and delays progression of osteoarthritis in animal models, it may serve as an optimum storage medium for OCAs. The objective of this study is to evaluate the effects of UPAL on cell viability and cartilage matrix status after 4-weeks storage of OCAs harvested from pig knees.
Methods and Materials
Pig knee OCAs (n=25, 4 mm in diameter, obtained<48h after sacrifice) were stored under the conditions as follows: (1) control group - stored in 1.5 mL complete medium (CM:DMEM/F12+10%FBS); (2) UPAL sol group - stored in 1.5 ml UPAL solution (1.2%) dissolved in CM; (3) UPAL gel group - cores encapsulated in 1.2% UPAL gel and stored in 1.5 ml CM. After 28 days of storage, the cartilage was removed from the bone and subjected to biochemical and histological analyses.
Results
Chondrocyte viability in the surface area of the UPAL gel group was significantly higher than that of the control group (p<0.05). Significantly higher proteoglycan (PG) synthesis was found in the UPAL gel group compared to the control group (p<0.05). Total nitric oxide (NO) production in the control group was significantly higher than that of the other groups (p<0.01). Cartilage in the UPAL gel group had significantly better histological scores than those of the control group (p<0.01).
Conclusion
It is well known that high chondrocyte viability in OCAs results in superior in vivo performance. The maintenance of cell viability and matrix content, as well as PG synthesis, in the alginate gel are also supported by the results of the low concentration of total NO in the storage medium. The use of UPAL gel encapsulation may be effective for improving OCA quality after 28 days of storage.
Presenter Of 1 Presentation
P172 - In Vitro Evaluation of the Effects of Ultra-purified Alginate on the Performance of Osteochondral Allografts Harvested from Pig Knees
Abstract
Purpose
Preventing degradation of osteochondral allografts (OCAs) before transplantation results in improvement of clinical outcomes after osteochondral allografting. Because ultra-purified alginate (UPAL) enhances cartilage repair and delays progression of osteoarthritis in animal models, it may serve as an optimum storage medium for OCAs. The objective of this study is to evaluate the effects of UPAL on cell viability and cartilage matrix status after 4-weeks storage of OCAs harvested from pig knees.
Methods and Materials
Pig knee OCAs (n=25, 4 mm in diameter, obtained<48h after sacrifice) were stored under the conditions as follows: (1) control group - stored in 1.5 mL complete medium (CM:DMEM/F12+10%FBS); (2) UPAL sol group - stored in 1.5 ml UPAL solution (1.2%) dissolved in CM; (3) UPAL gel group - cores encapsulated in 1.2% UPAL gel and stored in 1.5 ml CM. After 28 days of storage, the cartilage was removed from the bone and subjected to biochemical and histological analyses.
Results
Chondrocyte viability in the surface area of the UPAL gel group was significantly higher than that of the control group (p<0.05). Significantly higher proteoglycan (PG) synthesis was found in the UPAL gel group compared to the control group (p<0.05). Total nitric oxide (NO) production in the control group was significantly higher than that of the other groups (p<0.01). Cartilage in the UPAL gel group had significantly better histological scores than those of the control group (p<0.01).
Conclusion
It is well known that high chondrocyte viability in OCAs results in superior in vivo performance. The maintenance of cell viability and matrix content, as well as PG synthesis, in the alginate gel are also supported by the results of the low concentration of total NO in the storage medium. The use of UPAL gel encapsulation may be effective for improving OCA quality after 28 days of storage.