Introduction

Content

References

1. Loeser RF. Aging processes and the development of osteoarthritis. Curr Opin Rheumatol 2013;25(1):108-13. DOI: 10.1097/BOR.0b013e32835a9428.

2. Bullough PG. The role of joint architecture in the etiology of arthritis. Osteoarthritis Cartilage 2004;12 Suppl A:S2-9. DOI: 10.1016/j.joca.2003.09.010.

3. Hugle T, Geurts J, Nuesch C, Muller-Gerbl M, Valderrabano V. Aging and osteoarthritis: an inevitable encounter? J Aging Res 2012;2012:950192. DOI: 10.1155/2012/950192.

4. Martin JA, Buckwalter JA. Roles of Articular Cartilage Aging and Chondrocyte Senescence in Ostoarthritis. The Iowa Orthopedic Journal 2001;21:1-6.

5. Pippenger BE, Duhr R, Muraro MG, Pagenstert GI, Hugle T, Geurts J. Multicolor flow cytometry-based cellular phenotyping identifies osteoprogenitors and inflammatory cells in the osteoarthritic subchondral bone marrow compartment. Osteoarthritis Cartilage 2015;23(11):1865-9. DOI: 10.1016/j.joca.2015.07.021.

6. Collins JA, Diekman BO, Loeser RF. Targeting aging for disease modification in osteoarthritis. Curr Opin Rheumatol 2018;30(1):101-107. DOI: 10.1097/BOR.0000000000000456.

Introduction

Osteoarthritis (OA) is a destructive joint disease with high prevalence, representing a severe health burden and tremendous economic impact. Although cartilage destruction is the hallmark of OA, it is now appreciated that it is a whole joint disease involving changes in all joint tissues including articular cartilage, calcified cartilage, subchondral cortical and trabecular bone, joint capsule, ligaments, tendons, menisci, and the synovium (1,2). Any of these tissues may give rise to biomarkers as a consequence of joint disease, which may be detected in the synovial fluid, blood, and urine. The initiation of the disease can be attributed to direct injury and mechanical disruption of cartilage and other joint tissues, but the progressive changes are dependent on active cell-mediated processes that occur during the time course of the disease. Recent clinical observations and experimental studies have identified OA phenotypes that exhibit common profiles of symptoms, structural abnormalities, and pathophysiological pathways acting independently or in combination within a background of genetic and epigenetic factors (3,4). Multiple risk factors, including injury, aging, obesity, other metabolic disorders, and inflammation, impact on these OA phenotypes and may give insight into strategies for assigning biomarker panels that will stratify subsets of OA with distinct patterns of initiating factors and time courses of disease progression, but with similar end-stage pathology (5). Current therapies involve symptomatic relief of pain, physiotherapy, and ultimately total joint replacement after joint failure. The challenge in the search for disease modifying OA drugs (DMOADs) is that severe cartilage damage, as a critical determinant in OA progression, may not occur or may not be detected radiologically until months or years following the initiating event. Recent progress in identifying and validating biomarkers in at risk patients with early-stage disease holds great promise for impacting on the development of therapeutic approaches and the design of clinical trials.

Content

Efforts in the OA field have been focused on developing a biomarkers toolbox, based on experimental findings to enable understanding of mechanisms of pathogenesis and how they might be translated to therapy. Biomarkers are defined as surrogates for clinical endpoints that, in the case of OA, would substitute for outcomes such as pain, functional status, or candidacy for total joint replacement (6,7). While the importance of molecular biomarkers is increasingly recognized, no OA-related biomarker has been adopted for routine use in clinical practice, thus reflecting the lack of pharmacological structure-modifying DMOADs. Insights into the pathologic and pathogenic processes associated with the initiation and progression of OA have been provided by remarkable advances in defining the composition and structural organization of articular cartilage and in elucidating the molecular mechanisms regulating the anabolic and catabolic activities of chondrocytes. Much of the search for biomarkers, therefore, has focused on adult articular cartilage, which is a complex tissue of matrix proteins with a resident population of chondrocytes that are normally quiescent and maintain the matrix in a low-turnover state. Until recently, chondrocyte phenotypes were defined according to their developmental stage, for example, as proliferating, pre-hypertophic, and hypertrophic chondrocytes, or state of maturity, i.e., articular, and fibrocartilage chondrocytes. During OA, articular chondrocytes undergo phenotypic modulation, promoting matrix destruction, abnormal attempts at repair, and apoptotic cell death. Chondrocytes in aging cartilage, particularly those in the superficial zone, become senescent, possibly as a homeostatic protective mechanism, and release proteins involved in their senescence associated secretory phenotype (SASP). There are many inflammatory markers associated with SASP, which become more prominent when OA disease is present. Of the many cartilage biomarkers under investigation, the most consistent are related to matrix degradation such as the neo-epitopes created by enzymatic cleavage of collagen, aggrecan, and COMP (6). These OA-related events may be regulated by inflammatory mediators deriving from the synovitis in the joint, but also by the low-grade inflammation in the cartilage itself. While synovium is known to be heterogeneous with many cell populations such as fibroblasts, macrophages, and immune cells, recent evidence by single-cell RNAseq showed 12 distinct OA synovial cell phenotypes (9). In contrast, the dogma that cartilage contains one dominant cell type has been upended by RNAseq analyses showing 7 distinct OA chondrocyte phenotypes, particularly during OA progression (9, 10). The study of Chou et al. (9) also predicted the upstream mediators associated with these phenotypes in either synovial tissue or cartilage, supporting the concept that cross talk between synovial cells and cartilage plays critical roles in OA.

Similarly, there have been significant advances in characterizing the structural and functional alterations in periarticular bone in OA and in defining the factors and processes that control the remodeling and adaptation of skeletal tissues to local mechanical and systemic factors (2). Thus, products of bone resorption and formation have been added to the OA biomarker toolbox (6). Since the responses of bone cells to biomechanical damage in early stages of OA may be involved in cross talk with cartilage to initiate and promote damage, it will be important to include bone biomarkers in the toolbox.

Pre-clinical studies in vitro and in vivo have led to the development and testing of therapeutic agents and treatment interventions that have the potential to slow or attenuate OA progression, but significant challenges exist in translating them for clinical use. Advances in the development of advanced MRI technologies and biomarker assays that reflect changes in the synthetic and degradation products of the cartilage matrix and bone hold promise for early diagnosis, for assessing disease progression, and for more accurately monitoring of the efficacy of disease-modifying therapies; these, however, need further validation to be of clinical utility. Currently available pharmacological therapies address pain, which remains a major cause of disability in individuals with OA and further studies are needed to define the sources and mechanisms involved in the pathogenesis of joint pain and associated pain biomarkers to assess individual patients. Therefore, there is a great need for defining sets of biomarkers that will identify individuals who are at risk at early stages for more rapidly progressing to OA with the goal of developing structure modifying drugs that also ameliorate pain. At the very least, the biomarker subsets could be used to define cohorts of OA patients with different etiologies and risk factors to enable the design of more rational clinical trials for successful translation of phenotype-specific targeted therapies. The availability of appropriate biomarkers could enhance our ability to evaluate all stages and subsets of OA with regard to diagnosis, prognosis, drug development, and treatment. Overall, the validation of biomarkers as tools for monitoring disease status and patient responses to therapy is critical for facilitating drug development for OA.

References

1. Loeser RF, Goldring SR, Scanzello CR, Goldring MB. Osteoarthritis: a disease of the joint as an organ. Arthritis and rheumatism 2012;64:1697-1707.

2. Goldring SR, Goldring MB. Changes in the osteochondral unit during osteoarthritis: structure, function and cartilage-bone crosstalk. Nat Rev Rheumatol 2016.

3. Goldring MB, Berenbaum F. Emerging targets in osteoarthritis therapy. Curr Opin Pharmacol 2015;22:51-63.

4. Singh P, Marcu KB, Goldring MB, Otero M. Phenotypic instability of chondrocytes in osteoarthritis: on a path to hypertrophy. Ann N Y Acad Sci 2019;1442:17-34.

5. Favero M, Ramonda R, Goldring MB, Goldring SR, Punzi L. Early knee osteoarthritis. RMD Open 2015;1:e000062.

6. Kraus VB, Karsdal MA. Foundations of osteoarthritis section 3: clinical monitoring in OA chapter 15: biomarkers. Osteoarthritis Cartilage 2021.

7. Bay-Jensen AC, Mobasheri A, Thudium CS, Kraus VB, Karsdal MA. Blood and urine biomarkers in osteoarthritis - an update on cartilage associated type II collagen and aggrecan markers. Curr Opin Rheumatol 2022;34:54-60.

8. Favero M, Belluzzi E, Trisolino G, Goldring MB, Goldring SR, Cigolotti A, Pozzuoli A, Ruggieri P, Ramonda R, Grigolo B, Punzi L, Olivotto E. Inflammatory molecules produced by meniscus and synovium in early and end-stage osteoarthritis: a coculture study. J Cell Physiol 2019;234:11176-11187.

9. Chou CH, Jain V, Gibson J, Attarian DE, Haraden CA, Yohn CB, Laberge RM, Gregory S, Kraus VB. Synovial cell cross-talk with cartilage plays a major role in the pathogenesis of osteoarthritis. Sci Rep 2020;10:10868.

10. Ji Q, Zheng Y, Zhang G, Hu Y, Fan X, Hou Y, Wen L, Li L, Xu Y, Wang Y, Tang F. Single-cell RNA-seq analysis reveals the progression of human osteoarthritis. Ann Rheum Dis 2019;78:100-110.

Acknowledgments

I am grateful for research grant support during the later stage of my career and ecellent collaborations:

1. NIH-R01-AG022021: “ESE1 a novel transcriptional regulator of cartilage remodeling”

• Miguel Otero (Hospital for Special Surgery)

2. NIH-RC4 AR060546: “Defining Common Molecular Parameters for Onset and Progression of Osteoarthritis”

• Kenneth B. Marcu (Stony Brook University)

• Yefu Li (Harvard Medical School)

• Roland Wolkowicz (San Diego State Univ)

3. NIH-R21 AR054887: “Epigenetic Regulation of MMP-13”

• Richard Oreffo (Trudy Roach), et al. (Univ of Southampton)

• Ko Hashimoto (Tohoku Univ, Sendai, Japan)

4. Marjolein van der Meulen, PI (Cornell Univ): R21-AR064034: “Role of cartilage matrix in a model of load-induced OA”

5. Carl Blobel, PI (HSS): Arthritis Foundation IRG-6353: “Role of iRhom2 and ADAM17 in osteoarthritis”