Poster Cartilage /Cell Transplantation

P064 - Allogeneic Chondrocyte Transplantation with Demineralized Bone Matrix Scaffolds to Repair Cartilage Lesions: A Rabbit Study

Presentation Topic
Cartilage /Cell Transplantation
Date
13.04.2022
Lecture Time
09:30 - 09:30
Room
Exhibition Foyer
Session Name
7.3 - Poster Viewing / Coffee Break / Exhibition
Session Type
Poster Session
Speaker
  • A. Olivos Meza (Mexico City, MX)
Authors
  • A. Olivos Meza (Mexico City, MX)
  • J. Hernández León (Mexico City, MX)
  • C. Landa Solis (Mexico City, MX)
  • B. Olivos Díaz (Mexico, MX)
  • R. Gaytán (Mexico City, MX)
  • R. Pichardo Bahena (Mexico City, MX)
  • P. Silva Bermudez (Mexico City, MX)
Disclosure
No Significant Commercial Relationship

Abstract

Purpose

To regenerate hyaline cartilage like tissue using demineralized bone matrix scaffolds with allogenic chondrocytes in a rabbit model.

Methods and Materials

fig. 1 biosponge.jpg

fig. 2 biosponge.jpg

Allogenic chondrocytes were isolated and cultured from a donor rabbit. Chondrocytes were first mixed with chitosan (1: 1 proportion), this to promote cell adhesion when the chondrocytes were seeded in the DBM (5x5mm), at a density of 2x106 cells, the implants were kept in culture for two days to achieve cohesion. The cellular-scaffold was implanted in a grade-4 chondral lesion in the trochlear groove of the rabbit’s knee. At 12 weeks the rabbits were euthanized, by RT-PCR and immunofluorescence analysis looking for the expression of the SOX9, COL2A1 and aggrecan genes and histological stains. Subsequently, the samples were analyzed histologically with the modified O'Driscoll scale.

Results

Neocartilage was formed in both the cellular-scaffold and control empty group. However, the structural integrity, integration and bone regeneration in the repair tissue appeared to be much better in cell-scaffold group (Fig. 1) compared to the control group. Histologically, the formation of hyaline cartilage is carried out in the chondral lesions where the scaffold was implanted, which is confirmed by the O'Driscoll scale (Fig. 2). Similarly, positivity is produced by means of immunofluorescence for markers such as Collagen type 2, Agrecan and Sox-9, as well as the gene expression of SOX-9, COL2A1 and ACAN through PCR.

Conclusion

The DBM scaffold is a viable option, with sufficient chondrogenic potential for the formation of hyaline cartilage, which can make chondrocyte implantation a technique accessible to the population of developing countries.

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