Poster Cartilage and Meniscus

P106 - 3D Printed Meniscal Model for Meniscal Substitution: Study in Rabbits

Presentation Topic
Cartilage and Meniscus
Date
13.04.2022
Lecture Time
09:30 - 09:30
Room
Exhibition Foyer
Session Name
7.3 - Poster Viewing / Coffee Break / Exhibition
Session Type
Poster Session
Speaker
  • A. Olivos Meza (Mexico City, MX)
Authors
  • A. Olivos Meza (Mexico City, MX)
  • V. Cárdenas Soria (Mexico City, MX)
  • C. Landa Solis (Mexico City, MX)
  • P. Silva Bermudez (Mexico City, MX)
  • R. Gaytán (Mexico City, MX)
  • B. Olivos Díaz (Mexico, MX)
Disclosure
No Significant Commercial Relationship

Abstract

Purpose

To create a three-dimensional meniscus model by robotic impression with PCL enriched with either mesenchymal stem cells or fibrochondrocytes for implantation in rabbits.

Methods and Materials

Fibrochondrocytes (FC) and Mesenchymal Stem Cells (MSC) were harvested from remnants of meniscus and bone marrow of rabbits. Those cells were expanded for 14 days in monolayer, then were seeded on 3D printed meniscus models of polycaprolactone polymer and cultured during 4 days. The meniscus model was generated from Magnetic Resonance Imaging (MRI) data from human meniscus using a scale reduction according with the average of different measurements performed to lateral meniscus of 6 months age rabbits. Meniscal cell-scaffolds were used for partial substitution of lateral meniscus in 6 rabbits. Implants were leave during 12 weeks and new-formed tissue was harvested for histology, inmmunofluorescence, and RNA isolation studies.

Results

snap-121407-0012.jpgfig. 2 3d-meniscus.jpgMeniscal models showed calcein staining of either fibrochondrocytes and mesenchymal stem cells (Fig. 1). In the gross and histological analyses fibrocartilage like tissue was formed in both the FC and MSC groups but with better results in models seeded with fibrochondrocytes (Fig. 2). The presence of Col-1, Agrecane, and Col-2 has better expression in FC scaffolds compared to MSC group that showed osseous tissue formation. A third control acellular group also formed bone tissue as well as expressed RunX2 in RNA.

Conclusion

Is possible to form meniscus like tissue after 12-weeks of lateral 3D-printed meniscal substitution of PCL enriched with fibrochondrocytes

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