Podium Presentation Stem Cells

12.2.5 - Magnetic Nano Platforms for Enhancing Mesenchymal Stem Cells Chondrogenesis

Presentation Topic
Stem Cells
Date
13.04.2022
Lecture Time
16:57 - 17:06
Room
Potsdam 3
Session Type
Free Papers
Speaker
  • L. Labusca (Iasi, RO)
Authors
  • L. Labusca (Iasi, RO)
  • D. Herea (Bd Dimitrie Mangeron, RO)
  • A. Minuti (Iasi, RO)
  • C. Danceanu (Bd Dimitrie Mangeron, RO)
  • C. Stavila (Bd Dimitrie Mangeron, RO)
  • H. Chiriac (Bd Dimitrie Mangeron, RO)
  • N. Lupu (Bd Dimitrie Mangeron, RO)
Disclosure
No Significant Commercial Relationship

Abstract

Purpose

Nanotechnology is increasingly empowering the design of innovative methods for cartilage regeneration. Nanomagnetic materials can be used as versatile platforms for drug and bioactive molecules delivery, mechanical stimulation as well as for surface topography modification for the purpose of enhancing stem cell chondrogenesis. We report about the use of magnetic nanoparticles (MNPs) and magnetic nanowires (NW) and their role in adipose derived mesenchymal cells (ADSC) and Wharton Jelly derived mesenchymal stem cells (WJMSCs) chondrogenic conversion in vitro.

Methods and Materials

Human primary ADSCs and WHMSCs were loaded with proprietary bare iron oxide magnetic MNPs sized 10-50 nm by cell particle contact in culture media within a defined interval. Cell viability, proliferation as well as tri lineage differentiation (osteo, adipo and chondrogenesis) were performed. Chondrogenic pellets of ADSC and WJMSC loaded with MNPs were submitted to magnetomechanical stimulation within alternating magnetic field. Pellet histology and the amount of glycosaminoglycan deposition per cell was used to assess chondrogenic conversion. Cell viability and adherence on proprietary magnetic Nichel nanowires (Ni NW) was further tested. Chondrogenic assays were performed in suspension, pellet or in high density culture deposited on NW surfaces.

Results

Both ADSCs and WHMSCs displayed excellent viability and proliferative capability retaining their stemness compared to non-loaded controls. Significantly increased chondrogenesis was displayed by ADSCS-MNP under MF exposure but not by WJMSCs-MNPs. Cells were able to preserve viability in contact with MW, adhered at a relatively low rate on NW surfaces and were able to promote both cell type chondrogenesis.

Conclusion

Nanomagnetic platforms display attractive capabilities in improving ADSC chondrogenic conversion in vitro. MNPs and NW can be used to remotely control cell fate counting for future strategies of cartilage engineering

Acknowledgements

Financial support by the MCID NUCLEU Program (PN 19 28 01 01) and UEFISCDI Contract no. PCE20/2021 (PN-III-P4-ID-PCE-2020-2381) is gratefully acknowledged.

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