Poster Cartilage Imaging and Functional Testing

P014 - Establishment of a New Qualitative Evaluation Method for Early Articular Cartilage Injury by MRI Using a 17O-labeled Water as a Water Tracer

Presentation Topic
Cartilage Imaging and Functional Testing
Date
13.04.2022
Lecture Time
09:30 - 09:30
Room
Exhibition Foyer
Session Name
7.3 - Poster Viewing / Coffee Break / Exhibition
Session Type
Poster Session
Speaker
  • Y. Hosokawa (Sapporo, JP)
Authors
  • Y. Hosokawa (Sapporo, JP)
  • T. Onodera (Sapporo, JP)
  • K. Homan (Sapporo, JP)
  • K. Kudo (Sapporo, JP)
  • H. Kameda (Sapporo, JP)
  • H. Sugimori (Sapporo, JP)
  • N. Iwasaki (Sapporo, JP)
Disclosure
No Significant Commercial Relationship

Abstract

Purpose

In the early stages of cartilage damage, development of diagnostic methods focusing on the mechanism of maintaining hydrostatic pressure of cartilage is thoughted to be useful. On the other hand, 17O-labeled water, which is a stable isotope of oxygen, has the advantage of being free from radiation exposure and allergic reactions, and can be detected as the dynamics of water molecules by MRI. The purpose of this study is to evaluate MRI images using 17O-labeld water in a rabbit model.

Methods and Materials

Anterior cruciate ligament transection surgery was performed on 14-weeks-old male Japanese white rabbit. The lower legs were harvested at 4- and 8-weeks postoperatively, and MRI imaging, macroscopic and histological evaluation were performed. Using 3T MRI scanner, T2-weighted images using the 2D-fast spin echo method were performed repeatedly 18 times. 3 scans after the dynamic scanning, 17O-labeled water was manually administered via catheter. Multiple small regions of interest (ROI) were set in the articular cartilage of both condyles referring to the macroscopic or histologically apperance. Concentration of 17O in each phase of each ROI was calculated using the signal value of the T2-weighted image. The time course of concentration of 17O in each ROI was evaluated between the macroscopic and histological grades. All results were statistically compared using one-way ANOVA and significance was accepted with a p value < 0.05.

Results

An increase in novel contrast medium concentration was observed by MRI, consistent with macroscopic and histologically injured areas.
The macroscopic evaluation between the two groups showed that the concentration of 17O increased significantly after Phase 10 compared to the control area. Histologic evaluations also showed that the concentrations of 17O significantly increased in the group of OARSI grading 3 compared with that of other grades.

Conclusion

17O-labeled water could visualize earlier articular cartilage damage, which is difficult to detect by conventional methods.

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