P070 - Hyaluronic Acid Supports the Effects of Cortison and Avoids Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes in Vitro
- L. Moser (Krems, AT)
- L. Moser (Krems, AT)
- C. Bauer (Krems, AT)
- V. Jeyakumar (Krems, AT)
- E. Niculescu-Morzsa (Krems, AT)
- S. Nehrer (Krems, AT)
Abstract
Purpose
The current study aimed to investigate the chondrotoxicity of co-administrating local anesthetics (LA) with glucocorticoids (GC) and hyaluronic acid (HA) in vitro.
Methods and Materials
Human articular cartilage was obtained from five patients undergoing total knee arthroplasty. Chondrocytes were isolated, expanded and seeded in 24-well plates for experimental testing. Local anesthetics (Lidocaine 1%, Bupivacaine 0,5%, Ropivacaine 2%) were used separately and co-administered with each of the following substances: GC (Triamcinolone Hexacetonide (TH) 4,5mg/ml), GC plus HA (TH: 4,5mg/ml, HA: 22 mg/ml), HA (22mg/ml). Cells were incubated with the various conditions for one hour, before substances were discarded followed by two washing steps. Culture medium was added, and cells were incubated for 24 hours. After 24 hours microscopic images were taken, and metabolic activity was measured. Also, a viability assay using flow cytometry was performed.
Results
The addition of HA and GC/HA resulted in enhanced attachment and branched appearance of the chondrocytes compared to LA and LA/GC. Metabolic activity was better in all LA co-administered with HA and GC/HA than with GC and only LA (Figure 1). Flow cytometry revealed the lowest cell viability in lidocaine and the highest cell viability in ropivacaine (Figure 2). This finding was also confirmed by the live/dead assay.
Conclusion
In conclusion, HA supports the effect of GC and reduces chondrotoxic effects of LA in vitro. Thereby the co-administration of HA to LA and GC offers an alternative less chondrotoxic approach for treating patients with symptomatic osteoarthritis of the knee.