Purpose

Catwalk gait analysis system developed by Noldus records the paw prints of the rats walking in the band, they can classify them automatically and create dozens of parameters.

The purpose of this study is to evaluate whether the knees are completely healthy and that they are created chemically osteoarthritis with monosodiumiodoacetate in their knees firstly and then treated with intraarticular pharmacological agents such as hyaluronic acid, corticosteroid and astaxanthin.

Methods and Materials

In other studies, chemical osteoarthritis can be formed on animal models with different chemicals. In our study, monosodiumiodoacetate was preferred for chemical osteoarthritis in our experiment. We waited three weeks for chemical osteoarthritis to occur.

After the registration of the left knee of the rats with chemical osteoarthritis, their number was divided into 4 groups. Astaxanthin + hyaluronic acid to Group 1, astaxanthin to Group 2, steroid + hyaluronic acid to Group 3 and hyaluronic acid treatments to Group 4 were applied to the left hind knees with OA injection.

Waiting for four weeks after the treatment was expected to take effect. Four weeks later, the rats were re-run on the Catwalk device and the post-treatment values ​​were recorded.

Results

Results are automatically taken from the Catwalk gait analysis system

Conclusion

Catwalk device is a suitable method for the modeling of knee osteoarthritis in rats because it can record and automatically classify many data at the same time and allows comparison.

The monosodiumiodoacetate molecule produces effects similar to degenerative osteoarthritis disease by chondrolysis induced by cartilages, it is an appropriate method to create an experimental osteoarthritis model.

The astaxanthin molecule, which is in the experimental phase and which has a strong antioxidant properties in the treatment group, is a method that can be used as an alternative to the hyaluronic acid and steroid treatments currently in use in the treatment.