P. Neckar (Usti nad Labem, CZ)
Masaryk Hospital Usti nad Labem Sports medicine dep.Presenter Of 1 Presentation
18.4.1 - Evaluation of bone marrow MSCs from different sources for the treatment of chondral lesions of the knee in >40-year old patients
Abstract
Purpose
Concomitant application of mononuclear cells (MNCs) or cultured mesenchymal stem cells (MSCs) isolated from bone marrow and seeded on a 3D scaffold could increase the treatment efficacy in patients older than 40 years of age. The aim of our study was to assess two different sources of the bone marrow. Specifically, we compared the material obtained from the proximal tibia with the iliac crest (the commonly used bone marrow source) in terms of quantity and quality of the cells. Comparable features of the biological material would enable us to perform the bone marrow collection from the tibia perioperatively during the arthroscopic surgery. Such strategy would greatly reduce the burden of the patients in the treatment of the chondral lesions.
Methods and Materials
Bone marrow was collected using aspiration biopsy needles from the iliac crest and the proximal metaphysis of the tibia from ten volunteers older than 40 years of age during total knee replacement surgery. MNCs from the obtained material were isolated, cultured and phenotypic analysis was performed. Both sources were compared regarding the yield and viability of the MNCs as well as their ability for chondrogenic differentiation.
Results
The MNCs concentration/yield was significantly higher in the samples from the iliac crests. Similar results were obtained with the cultured MSCs after the first passage when the MSCs/MNCs ratio was compared. Nevertheless, the qualitative analysis that included MSC immuno-phenotyping, viability and population doubling time showed no difference between the two tested bone marrow sources.
Conclusion
The iliac crest represents a superior bone marrow source as for the MNCs and MSCs yield however there was no qualitative difference between the isolated and cultured cells. The population doubling time analysis showed that the tibia is a good alternative source of the MSCs which can be obtained at therapeutically relevant number for the treatment of chondral lesions of the knee.