Z. Shao (Beijing, CN)

Peking University Third Hospital Institute of Sports Medicine

Presenter Of 1 Presentation

Podium Presentation Biomaterials and Scaffolds

16.3.7 - Surface modification on biomaterials with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering

Presentation Number
16.3.7
Presentation Topic
Biomaterials and Scaffolds
Lecture Time
12:09 - 12:18
Session Type
Free Paper Session
Corresponding Author

Abstract

Purpose

Synovium-derived mesenchymal stem cells (SMSC) have been studied for over a decade since first being successfully isolated in 2001. These cells demonstrate the most promising therapeutic efficacy for musculoskeletal regeneration of the MSC family, particularly for cartilage regeneration. However, the mobilization and transfer of MSCs to defective or damaged tissues and organs in vivo with high accuracy and efficiency has been a major problem in tissue engineering (TE).

Methods and Materials

In the present study, we identified a seven amino acid peptide sequence [SMSCs-affinity peptide (LTHPRWP; L7)] through phage display technology that has a high specific affinity to SMSCs. Thereafter, L7 was covalently conjugated onto both polycaprolactone (PCL) electrospun meshes and human decalcified bone scaffolds (hDBSc) to investigate its TE applications.

Results

Our analysis suggested that L7 efficiently and specifically interacted with SMSCs without any species specificity. After 24 h coculture with human SMSCs (hSMSCs), L7-conjugated PCL electrospun meshes had significantly more adherent hSMSCs than the control group, and the cells expanded well. Similar results were obtained using hDBSs. These results suggest that the novel L7 peptide sequence has a high specific affinity to SMSCs. Covalently conjugating this peptide to either artificial polymer material (PCL mesh) or natural material (hDBS) significantly enhances the adhesion of SMSCs.

Conclusion

This method is applicable to a wide range of potential SMSC-based TE applications, particularly to cartilage regeneration, via surface modification on various type of materials.
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Meeting Participant of

Turner - ICRS Board Room (15) ICRS Committee Meeting

Fellowship / Grant Committee

Lord Byron - ICRS Meeting Room (20) ICRS Committee Meeting

Communication Committee (New Committee19-21)

Session Type
ICRS Committee Meeting
Date
07.10.2019
Time
13:00 - 14:00
Location
Lord Byron - ICRS Meeting Room (20)