R. Tomaszewski (Katowice, PL)

MEDICAL UNIVERSITY OF SILESIA Department of Trauma and Orthopaedic Surgery

Presenter Of 1 Presentation

Podium Presentation Cartilage /Cell Transplantation

18.3.9 - The results of cartilage damage treatment using growth plate chondrocytes on the growing animal model.

Presentation Number
18.3.9
Presentation Topic
Cartilage /Cell Transplantation
Lecture Time
15:27 - 15:36
Session Type
Free Papers
Corresponding Author
Disclosure
No Significant Commercial Relationship

Abstract

Purpose

Due to the specific structure and highly specialized function, treatment of articular cartilage damage is a major clinical problem. Moreover its avascular character results in low regenerative capacity. The aim was to compare the results of treatment of cartilage damage treated using scaffold , bone marrow, and growth plate chondrocytes.

Methods and Materials

The study group consisted of 10 pigs at the age of 12 weeks. a bone marrow biopsy was performed from the posterior iliac spine. We divided the scaffolds into two groups. First one consisted scaffold with bone marrow and growth plate chondrocytes. Second group contended only of scaffolds with bone marrow without chondrocytes . The samples from pig’s knee according to ICRS classifications was evaluated after 12 weeks from the day of surgery.

Results

Macroscopic evaluation according to ICRS classification, in the group of pigs treated with scaffolds without chondrocytes, all animals showed nearly normal morphology with an average of 9.66/ 12 points. In the group of pigs treated with scaffolds incubated with chondrocytes, all animals similarly showed nearly normal morphology with an average of 10.44/ 12 points. Based on microscopic evaluation, 1 very good results and 8 good results were obtained in the group of pigs treated with scaffolds without cells, with an average of 70.44%; in the group of pigs treated with the scaffolds incubated with the cells, 5 very good results and 4 good results were obtained with an average of 79.61%.

Conclusion

1. Treatment of joint cartilage damage with the use of additional growth cartilage chondrocytes improves the quality of joint cartilage healing process.

2. Chondrocyte clustering is a manifestation of the cartilage repair process starting from the side of the stimulated subchondral bone.

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