V. Mantripragada (Cleveland, US)
Cleveland Clinic Biomedical ENgineeringPresenter Of 1 Presentation
18.2.2 - Progenitors from Cartilage, Bone Marrow, Infrapatellar fat pad, Synovium, Periosteum – A Quantitative Comparison for Cartilage Repair
Abstract
Purpose
Cell-based therapy for cartilage repair is becoming an established technique for modern healthcare. Unfortunately, there is no consensus on an optimal cell source yet. The study provides a donor-matched quantitative comparison of the connective tissue progenitors (CTPs) derived from cartilage (Outerbridge grade G1-2-3), bone marrow aspirate concentrate (BMC), infrapatellar fat pad (IPFP), synovium (SYN) and periosteum (PERI) with respect to: 1) cell concentration (cells/cc), 2) CTP prevalence (colony forming units per million cells), and 3) biological performance based on in vitro proliferation (cells per colony), migration (colony density) and differentiation (expression of negatively charged extracellular matrix: GAG-ECM) potentials.
Methods and Materials
Ten patients undergoing total knee arthroplasty (mean-age: 59years, females=6) were recruited. Automated ASTM-based quantitative colony forming unit assay was used to compare cell concentration, CTP prevalence, and biological performance across tissue sources. A significance level of 0.05 was used overall. Tukey’s multiple comparison method was used to exam all pairwise comparisons between cell sources.
Results
Cell concentration was highest in G3 (p=0.002) and BMC (p=0.001). CTP prevalence was highest in IPFP (p=0.001), SYN (p=0.003) and G1-2 cartilage (p=0.02). Proliferation potential was highest in SYN (p<0.001) derived CTPs. Migration potential was highest in G1-2-3 (p<0.001). Differentiation potential was highest in G1-2-3 (p<0.001). CTPs derived from any given tissue sources and any given individual were highly heterogeneous in biological performance.
Conclusion
Data presented in this study suggests that cartilage (G1-2-3) is the preferred tissue source for cartilage repair, based on PCTP and GAG-ECM, followed by SYN, IPFP, BMC and PERI. However, within each tissue source, there exists a subset of CTPs with biological performance similar to G1-2-3 cartilage, particularly in SYN and IPFP. Refinements to current cell expansion strategies, particularly performance-based clonal selection and expansion of preferred CTPs and their progeny can potentially lead to improved cell population with predictive future.