P006 - Tissue adhesive patch – cartilage (TAP-C): A glue improve osteochondral grafts transplantations for joint repair application.
Abstract
Purpose
Autologous or allogenous osteochondral graft would be an important method to treat an extensive osteochondral defect. The success of transplantation is influenced by how well the donor cartilage and bone are integrated into the recipient cartilage and bone, respectively. In the previous study, a highly adhesive and gel-like engineered cartilage tissue was fabricated from human cartilage progenitor cells. In this study, its application to osteochondral graft is intended to enhance the integration of donor and recipient tissues.
Methods and Materials
Adhesive cartilage patch (TAP-C) was created by culturing the human cartilage progenitor cells. Chemical components of TAP-C were provided by biochemical analysis (DNA, Glycosaminoglycan, collagen and protein content). The potential of chondrogenic and osteogenic differentiation of TAP-C was also observed. This study has 3 groups: i) Group1: untreated; ii) Group2: treated of Fibril Glue; iii) Group3: treated of TAP-C. The results would be evaluated at 0, 1, 2 and 4 weeks in vitro, 0, 4, 8, 12 weeks ex vivo, 4, 8 and 12 weeks for the rabbit model, 3 and 6 months for the goat model.
Results
TAP-C had chemical components of cartilage and was differentiated into bone and cartilage tissue. In vitro and ex vivo experiments showed that the cartilage-bone adhesive strength in TAP-C treated group was higher than that of fibrin glue treated group and untreated group. Fibrin glue treated group displayed no significant difference in results measured in the untreated group. For in vivo experiment, results showed that the TAP-C treated group was better than fibrin glue treated group, and both were better than the untreated group.
Conclusion
This study investigated that TAP-C could be a good tissue glue for cartilage and bone binding. The cells in TAP-C strengthen the integration between two tissues by migration into the recipient cartilage and bone tissue and by differentiation into bone and cartilage tissue.
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