P171 - Effect of Placenta-Derived Mesenchymal Stem Cells and Hyaluronic Acid Composite on Articular Cartilage Regeneration in a Rabbit Model
Placenta-derived mesenchymal stem cells are attracting great interest as a promising cell source for regenerative medicine due to their non-invasive collection, ease of use, high expansion capacity and low immunogenicity. We sought to investigate the possibility of placental-derived MSC and hyaluronic acid (HA) hydrogel composite to treat full-thickness articular cartilage defects . We also investigated the effects of various concentrations of placenta-derived MSC and HA hydrogel composite on cartilage regeneration effects in rabbit model.
Methods and Materials
Critical-sized osteochondral defects (5mm diameter × 5mm depth) were created in the center of the trochlear groove of femur. The Four experimental groups were as follows: group A, defect only (left knee)/ HA hydrogel without placenta-MSCs (right knee), n=6; group B, 0.01 x 107 cells/ml with HA hydrogel (left knee)/ 0.1 x 107 cells/ml with HA hydrogel (right knee), n=8; group C, 0.1 x 107 cells/ml with HA hydrogel (left knee)/ 0.5 x 107 cells/ml with HA hydrogel (right knee), n=8; group D, 0.5 x 107 cells/ml with HA hydrogel (left knee)/ 1 x 107 cells/ml with HA hydrogel (right knee) were transplantation in a rabbit model. At 8 weeks after transplantation, the degree of cartilage repair was evaluated grossly and histologically using the International Cartilage Repair Society macroscopic score, the modified O’Driscoll score for histologic grading.
Overall, the group transplanted with placental-derived MSCs and HA hydrogels showed better cartilage regeneration in the HA group or defect group. Interestingly, The 0.5 x 106 cells/ml group showed the highest cartilage repair at 8 weeks post transplantation.The 0.5 x 106 cells/ml group showed the highest cartilage repair at 8 weeks post transplantation.
The results of this study suggest that composite transplantation of Pla-MSCs and HA is beneficial for cartilage therapy. In addition, this study shows that optimal MSC concentrations need to be determined for better cartilage repair.