10.3.8 - Can osteoarthritis biomarkers monitor clinical improvement?
- E. Antonioli (São Paulo, BR)
- E. Antonioli (São Paulo, BR)
- R. Ravagnani-Campedelli (São Paulo, BR)
- F. Bruno Dias De Oliveira (Sao Paulo, BR)
- S. Agarwal (Sao Paulo, US)
- M. Ferretti (São Paulo, BR)
Abstract
Purpose
There is no cure or disease modifying drugs available to treat osteoarthritis (OA). Therefore, effective biomarkers and diagnostic tests are direly needed to monitor the disease progression and treatment. The objective of this study is to identify and evaluate mediators that serve as biomarkers of OA progression and treatment.
Methods and Materials
The rehabilitation protocol for patients with mild to moderate knee OA consisted of muscle strengthening exercises activating: hamstrings, quadriceps, and gluteal muscles, during 8 weeks (3 session/week). The efficacy of protocol was evaluated by functional scale (WOMAC), quality of life questionnaire (EuroQol), pain scale (VAS), and physical function tests (TUG, isokinetic dynamometer). Serum levels of COMP (cartilage oligomeric matrix protein), CS846 (aggrecan neoepitope), C2C (cleavage of type II collagen), IL-6,-8 and-10, and, HMGB1 (High mobility group box 1) were evaluated by ELISA. All outcomes were performed before and after the rehabilitation program. A correlation of physical improvement and serum biomarkers was performed. Statistical analyzes were performed by Student's t-Test, one/two-way ANOVA and Pearson's Correlation.
Results
Thirty six individuals (23 women and 13 man) with moderate knee OA (KL 2-3; age: 55.1 ± 5.29 years; BMI: 28.9 ± 3.8 Kg/m2; Mean ± SD), were evaluated. After the treatment, the subjects reached 50% reduction in the WOMAC scale, significantly showing an improvement in pain, stiffness, mobility, and functional test performance. And also an increased maximal strength production and maximal strength lift capacity for the knee extensor and flexor muscles and improved muscle balance. There was no significant difference in levels of serum biomarkers after the treatment and no evidence of correlation between biomarkers serum levels and clinical improvement.
Conclusion
Physical exercise resistance was sufficient to reduce pain, increase strength production, improve functional performance and muscle balance of OA subjects. However, biomarkers do not present evidence to monitor clinical improvement after conservative treatment.