Podium Presentation Stem Cells

18.2.4 - Nanosecond Pulsed Electric Fields (nsPEFs) Enhanced Stemness of Mesenchymal Stem Cells via downregulation of DNMT1

Presentation Number
18.2.4
Presentation Topic
Stem Cells
Lecture Time
14:42 - 14:51
Session Type
Free Papers
Corresponding Author
  • Z. Ge (Beijing, CN)
Authors
  • K. Li (Beijing, CN)
  • Z. Ge (Beijing, CN)
Disclosure
Current research is financially supported by NSFC grant 81772334.

Abstract

Purpose

Upregulation of differentiation potential of mesenchymal stem cells contributes to the development of regenerative medicine. Nanosecond pulsed electric fields (nsPEFs) have been shown to influence intracellular organelles and trigger strong biological effects [1, 2]. In current study, we study the effects of nsPEFs on differentiation potential of pig MSCs (Mesenchymal stem cells) in vitro and explore the mechanisms behind the phenomenon.

Methods and Materials

The effects of nsPEFs-preconditioning on the expression levels of tri-lineage differentiation were evaluated by quantitative RT-PCR of marker genes and Histological staining. Mechanisms were explored with gene expression level of OCT4 and NANOG and the methylation level of their promoter. The expression of DNA methylation transferase and global DNA methylation level were performed to further study the mechanisms behind.

Results

nsPEFs-preconditioning with proper parameters (10 ns at 20 kV/cm, 100 ns at 10 kV/cm) significantly potentiated tri-lineage differentiation capacity of MSCs with upregulated genes expression(Fig.1). nsPEFs-preconditioning promotes the formation of glycosaminoglycan for chondrogenic differentiation, calcium nodule for osteogenic differentiation, lipid droplet for adipogenic differentiation through demethylation of OCT4 and NANOG promoter and upregulation of OCT4 and NANOG, followed by downregulation of DNMT1 and global DNA methylation level (Fig.2).

abstract-fig1.pngabstract-fig2.png

Conclusion

This study demonstrates a unique approach of nsPEFs-treatment to potentiate the tri-lineage differentiation potential of MSCs through demethylation of OCT4 and NANOG which has translational potential for MSCs-based regenerative medicine.

Reference

1. Zhang, K., et al., Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/β-catenin Signaling Pathway. Scientific Reports, 2014. 4: p. 5836.

2. Ning, T., et al., Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway. Stem cell research & therapy, 2019. 10(1): p. 45.

Collapse