Author Of 1 Presentation
NEW MODELS FOR FLASH STUDIES.
Abstract
Background and Aims
Currently, many research groups are more interested in the FLASH radiotherapy characterized by irradiation with ultra-high dose rate. A first usual step is to validate the beam line for FLASH studies by reproducing published FLASH effect in animals. However, it classically requires time consuming animal studies with dedicated skills, authorizations and infrastructures. Thus, to provide alternative methods and facilitate the implementation and validation of new FLASH beams, we aimed at developing in vitro and ex vivo models that will allow rapid and pertinent evaluation of the FLASH effect.
Methods
For our studies, we are using the ElectronFLASH LINAC manufactured by SIT company. To achieve this goal, we first used an in vitro model of human lung basal stem cells obtained from patients. Cultured in specific air-liquid conditions, this model allows the monitoring of stem cells survival and their capacity to differentiate after irradiation. In parallel, we adapted organotypic lung slices model, recapitulating lung complexity, architecture and microenvironment interactions, for radiation toxicity studies.
Results
Our results indicate that organotypic lung slices enables a rapid evaluation of the FLASH effect.
Conclusions
These models developed in the lab allow to rapidly determine the impact of the various beam parameters on FLASH effect with a robust and reproducible assay. With the inclusion of tumoral cells within the organotypic lung slices, we hypothesize that this ex vivo model can assess concomitantly the FLASH sparing effect on healthy tissue as well as the antitumoral efficacy. Moreover, the model can apply for human patient samples as well as rodent tissues.