Valentina Benfenati, Bologna, ItalyConsiglio Nazionale delle Ricerche Institute for Organic Sinthesys and Photoreactivity
Presenter Of 1 Presentation
Multiscale excitation and recording of astrocytes signalling and conductance by nanostructured devices
The past four decades demonstrated that the expression and function of proteins forming ion channels, receptors and water channels in astrocytes are critical in brain function & dysfunction. However, most of the tools and approaches used to probe and sense astrocytes are derived from those developed to study neurons. The goal of our research is to provide insight on astrocytes physiology and biophysics by using multifunctional materials and nanotechnological approaches targeted to record and manipulate structural and functional properties of ion channels, calcium signalling and water permeability. The presentation will show the results of our recents works demonstrating that 1)the growth on nano-biomaterials induces astrocytes differentiation that is accompanied with upregulated expression at the process microdomains and increased function of K+channel Kir4.1 and Aquaporin-41; 2)the use of carbon-based and silicon based device enable the electrical stimulation of intracellular Ca2+([Ca2+]i) and the extracellular recording of transmembrane voltage oscillations in the slow frequency range in differentiated astrocytes in vitro2; 3) infrared laser pulse provide the possibility to stimulate water transport, [Ca2+]iand whole-cell conductance mediated via Transient Receptor Potential Ankirin 1 (TRPA1) and TRP Vanilloid-4 (TRPV4)3.
Acknowledgments:The work is supported by AFOSR-Biophysics Programme with the projects ASTROMAT, ASTRONIR, 3D NEUROGLIA. The speaker is grateful to all the co-authors and Co-PIs that contributed to the results presented.1Saracino E., Maiolo L., et al., Adv Biosystem, 2020, in press; Saracino, Guarino et al., J Mater Chem B 2Borrachero-Conejo et al., Adv Healt. Mater, 2020 3Borrachero-Conejo A., Adams W. et al., Faseb J, under review