Sebastian Haesler, Leuven, BelgiumNeuroelectronics Research Flanders NERF
Presenter Of 1 Presentation
Chronic large-scale recording with Neuropixels 2.0 probes
11:00 - 14:00
11:00 - 11:30
Abstract BodyHow dynamic activity in neural circuits gives rise to behaviour is a central area of interest in neuroscience. A key experimental approach for addressing this question involves measuring extracellular activity of neurons in rodent animal models performing behavioural tasks. Recently developed Neuropixel probes have become a key enabling technology for measuring neural activity in large tissue volumes with high spatiotemporal resolution. Here, we report extracellular recordings obtained with the next-generation Neuropixel 2.0 probes. These probes have a smaller base PCB which facilitates their chronic use in mice. Moreover, in addition to the 1-shank design, a 4-shank probe has been fabricated. As in the previous version, 384 channels can be recorded simultaneously. To allow for performing repeated measurements of neural activity day after day, we have developed a fixture for chronic probe implantation. The fixture is composed of a probe mount to which the probe is permanently attached and which is reversibly mounted on a probe base, which is implanted on the animal. At the end of an experiment, probes can be safely recovered for re-implantation in another experimental animal by removing the probe mount from the probe base, which remains on the animals’ skull. We implanted up to two 4-shank probes in the olfactory cortex of mice and recorded singe-unit responses to odorant stimulation over weeks. Taken together, we believe Neuropixel 2.0 probes provide a key tool for enabling routine chronic recordings, overall increasing the resolution and reliability of in vivo electrophysiology experiment.
Moderator Of 1 Session
11:00 - 14:00
The workshop will highlight the measurement capabilities of key technologies for large-scale monitoring of neural activity, in particular fully integrated Neuropixels silicon probes and wide-field 2-photon imaging. We have selected leading experts in the field as speakers to share recent data and protocols related to all technical aspects; from building setups, to data processing (spike sorting and image processing), storage and analysis. The workshop also aims at stimulating discussions about major challenges, in particular the analysis of large datasets and the scientific interpretation of brain-wide signals in the context of behavior. Finally, we will discuss current technical limitations and possible improvements in next generation technologies. The purpose of the workshop is to bring together the rapidly growing user community and to establish best-practices in order to ensure data quality is maintained at the larger scale of measurement. The workshop further aims at facilitating the adoption of new technologies by lowering the threshold to become first-time users. Taken together, it will advance the technical capabilities of the circuit neuroscience community and it will foster scientific exchange related to a major question in neuroscience – how the coordinated neural activity across brain regions gives rise to mental function and behavior.