Luca Pierri (Italy)

University of Naples, Federico II Pediatrics
I am a young physician specializing in pediatrics in 2021 with a thesis in Pediatric Infectious Diseases, focusing on Coronavirus Disease 2019. During my training I was able to focus my attention on Pediatric and Neonatal infectious diseases, the use of lung ultrasound in the pediatric and neonatal setting and the role of metabolomics in describing the pathophysiological processes underlying some obesity-related hepatometabolic diseases.

Author Of 1 Presentation

LONGITUDINAL ANALYSIS OF METABOLOMIC SERUM SIGNATURE IN PEDIATRIC PATIENTS WITH SARS-COV-2 INFECTION AND MIS-C PATIENTS COMPARED TO HEALTHY CONTROL.

Date
Wed, 11.05.2022
Session Time
13:40 - 15:10
Session Type
Joint Symposium
Room
BANQUETING HALL
Lecture Time
14:42 - 14:50

Abstract

Backgrounds:

Metabolomic alterations have been identified in adults with SARS-CoV-2 infection, however this approach has not been used in children so far. Children usually have a mild course, although a small percentage may develop severe disease or Multisystem Inflammatory Syndrome (MIS-C).

Methods

We carried out a prospective comparative cohort study (April 2020 to June 2021) enrolling children referred to our COVID-center for symptoms related to acute SARS-CoV-2 infection (positive nasopharyngeal swab) or MIS-C and a cohort of age- and sex-matched children who served as controls. Metabolomic analysis was performed by Gas Chromatography Mass Spectroscopy approach using blood samples collected at admission, acute phase, discharge and a follow-up visit scheduled after negativization. All enrolled patients were hospitalized and classified into mild-to-moderate or severe COVID-19 according to clinical, radiological, and biochemical features.

Results:

figure abstract1.jpgA specific metabolomic signature was identified in 92 children (48 males, mean age 3.69±5.1 years) with acute SARS-CoV-2 infection, compared to 41 controls (permutation test statistic p=0.0015, Figure) involving specific pathways, such as: inflammation (spermidine and hypoxanthine), reactive oxygen species pathway (riboflavin) and glicerolipids pathways.

Distinct metabolic signatures were significantly associated with child’s age (mainly > 3 years), clinical and biochemical severity and timing from SARS-CoV-2 infection.

Children with MIS-C (n=9) showed a unique metabolomic signature and different from age- and sex-matched SARS-CoV-2-infected patients or controls characterized by an alteration of spermidine and sphingolipids.

Conclusions/Learning Points:

Pediatric SARS-CoV-2 infection has a characteristic metabolomic signature suggesting a possible involvement of intestinal microbiome, that varies according to patients’ age and disease phenotype. Metabolomic approach may be a useful tool to identify possible early markers of disease and predictors of severe diseases evolution or multi-system inflammatory syndrome.

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Presenter Of 1 Presentation

LONGITUDINAL ANALYSIS OF METABOLOMIC SERUM SIGNATURE IN PEDIATRIC PATIENTS WITH SARS-COV-2 INFECTION AND MIS-C PATIENTS COMPARED TO HEALTHY CONTROL.

Date
Wed, 11.05.2022
Session Time
13:40 - 15:10
Session Type
Joint Symposium
Room
BANQUETING HALL
Lecture Time
14:42 - 14:50

Abstract

Backgrounds:

Metabolomic alterations have been identified in adults with SARS-CoV-2 infection, however this approach has not been used in children so far. Children usually have a mild course, although a small percentage may develop severe disease or Multisystem Inflammatory Syndrome (MIS-C).

Methods

We carried out a prospective comparative cohort study (April 2020 to June 2021) enrolling children referred to our COVID-center for symptoms related to acute SARS-CoV-2 infection (positive nasopharyngeal swab) or MIS-C and a cohort of age- and sex-matched children who served as controls. Metabolomic analysis was performed by Gas Chromatography Mass Spectroscopy approach using blood samples collected at admission, acute phase, discharge and a follow-up visit scheduled after negativization. All enrolled patients were hospitalized and classified into mild-to-moderate or severe COVID-19 according to clinical, radiological, and biochemical features.

Results:

figure abstract1.jpgA specific metabolomic signature was identified in 92 children (48 males, mean age 3.69±5.1 years) with acute SARS-CoV-2 infection, compared to 41 controls (permutation test statistic p=0.0015, Figure) involving specific pathways, such as: inflammation (spermidine and hypoxanthine), reactive oxygen species pathway (riboflavin) and glicerolipids pathways.

Distinct metabolic signatures were significantly associated with child’s age (mainly > 3 years), clinical and biochemical severity and timing from SARS-CoV-2 infection.

Children with MIS-C (n=9) showed a unique metabolomic signature and different from age- and sex-matched SARS-CoV-2-infected patients or controls characterized by an alteration of spermidine and sphingolipids.

Conclusions/Learning Points:

Pediatric SARS-CoV-2 infection has a characteristic metabolomic signature suggesting a possible involvement of intestinal microbiome, that varies according to patients’ age and disease phenotype. Metabolomic approach may be a useful tool to identify possible early markers of disease and predictors of severe diseases evolution or multi-system inflammatory syndrome.

Hide