Recently, the impact of the gut microbiota on human health has become more apparent. While bacterial communities are widely studied, the fungal research lags behind. This study aims to analyze both bacterial and fungal microbiota composition in CVID disorders.
A group of 16 case-control couples living in the same household provided stool samples for gut microbial profiling using targeted 16S and ITS rRNA amplicon sequencing (Illumina). Data were analyzed and visualized using QIIME pipeline, R software and online tool Calypso.
We revealed that CVID patients harbor less diverse and significantly changed bacterial but not fungal microbiota communities in their gut. Further, the same household factor strongly impacted our data. Individuals in couples shared more similar microbiota with each other than with strangers, and thus using paired case-control samples could provide a better resolution between disease-related dysbiosis and other environmental confounders. In the case of bacterial analyses, the household impact did not outweigh the influence of health status indicating the bacterial role in CVID pathology. In the case of fungal analyses, the household was the most significant diversity determining factor, while the influence of the health status was not reflected in fungal data suggesting that fungal contribution to CVID phenotype may not be relevant.
We suppose that bacterial microbiota dysbiosis in CVID disorders might have a biological relevance, which is not probably true for fungal microbiota.
Supported by: MUNI/M/1322/2015/; MUNI/A/1298/2018; CKTCH IG201802