Daniela Ababei, Romania
Facultatea de Medicina si Farmacie Grigore T. Popa PharmacologyAuthor Of 2 Presentations
The effects of Camelina sativa seed extract in a complex irritable bowel syndrome mice model, focussing on preliminary correlations between lipid peroxidation and behavioral parameters - EPV0767
Abstract
Introduction
Irritable bowel syndrome (IBS) is one of the most frequent functional gastrointestinal disorders. The multifactorial approach suggests that oxidative stress is a major component in IBS development. Considering the multifaceted mechanisms and dysregulations occurring in IBS, a possible interaction between central nervous system and gastrointestinal tract could partially explain the IBS symptoms modulation through physiological and psychological stress in animal models.
Objectives
In this way, we previously described the possible implications of cognitive and oxidative stress impairments in IBS. In this study, we aimed to evaluate the antioxidant potential of Camelina sativa seeds extract and the possible interaction between the oxidative and behavioural changes in a complex IBS mice model.
Methods
Neonatal mice experienced maternal separation (PN1-14), contention stress (PN90-92) and multifactorial stress (PN90-95). Camelina sativa extract was administered (PN98-101). Following behavioural assessment, brain and bowel tissues were collected and subjected to biochemical assessment (thiobarbituric acid-reactive substances determination).
Results
Camelina sativa extract administration lead to decreased MDA levels, as compared to control group (p<0.05). Furthermore, linear regression statistical analyses showed correlations between lipid peroxidation marker and some behavioural parameters. In this way, we observed that Camelina sativa seeds extract could exhibit antioxidant potential in a complex IBS mice model. Moreover, it seems that the oxidative stress changes could be interacting with the behaviour, in the context of Camelina sativa seeds extract administration.
Conclusions
Our study provides additional evidence that Camelina sativa seeds extract could exhibit antioxidant potential in a complex IBS mice model. Furthermore, we observed that the oxidative stress and behavioural changes could be correlated.
The preliminary results regarding the effects of Camelina sativa extract in a complex irritable bowel syndrome model on mice - EPV1196
Abstract
Introduction
Irritable bowel syndrome (IBS) is one of the most common gastrointestinal disorder. Lacking a reliable biomarker, IBS is best understood from a biopsychosocial perspective. IBS mechanisms involve a dysregulation in interactions among the cognitive and emotional centers of the central nervous system. Psychological stress is an element that links factors influencing the gut-brain connection triggering IBS symptoms.
Objectives
Our aim was to develop a reliable and effective IBS model that would result in the emotional depressive changes accompanying the digestive disturbances. Also, investigating the therapeutic potential of Camelina sativa seeds oxidative properties in mice model of IBS, focusing on antioxidant enzymes and behavioral parameters.
Methods
Neonatal mice (n=12) experienced maternal separation (postnatal days 1-14), 3 days contention stress (postnatal days 90-92). Half of the group was exposed to six additional different stressors (postnatal days 90-95). Postnatal days 98-101, Camelina sativa extract was administered. Y-maze task, elevated plus maze and forced swimming test were used for assessing behavioural parameters. Brain and colon tissues were collected and subjected to biochemical assessment (glutathione peroxidase- GPx, superoxide dismutase-SOD).
Results
We observed changes in oxidative stress status in brain as compared to colon. Beneficial effects of Camelina sativa seeds in alleviating behavioral deficits in IBS model. An increased enzymatic activity of SOD in brain tissue in neomaternal separation and contention stress exposed groups given by the effect of Camelina sativa extract.
Conclusions
There are changes in the status of oxidative stress, it was demonstrated and highlighted in the central nervous system, without being fully replicated in the colon yet.