Jennifer Härdfeldt (Sweden)
Karolinska Institutet Metabolism Unit, Endocrinology, Metabolism and Diabetes, Department of Medicine,Author Of 1 Presentation
O018 - Interstitial postprandial hyperlipidemia and remnant lipoproteins in type 2 diabetes mellitus (ID 768)
Abstract
Background and Aims
Diabetic dyslipidemia is characterized by elevated fasting serum TG and VLDL and low HDL-c. Postprandial hypertriglyceridemia and changes in remnant-like lipoprotein particles are well recognized in T2D, but the metabolism and /or clearance of these triglyceride-rich lipoproteins (TGRL) is less clear. The interstitial fluid (IF) represents the immediate environment of most cells in the body and studying postprandial changes in this compartment should provide novel insights into the increased cardiovascular risk observed in T2D.
Methods
Five T2D patients with adequate control of diabetes and matched healthy controls were given a standardized meal (1270 kcal; 70/31g fat/saturated fat; 96/60g carbohydrates/sugar, 64g protein) after fasting overnight. Serum was collected hourly for 9h following meal, and IF was harvested through skin blisters at 3 timepoints (TP1=0-3h, TP2=3-6h, TP3=6-9h). Analyses of serum and IF TG, TGRL and lipoprotein lipids were determined by FPLC and ELISA.
Results
T2D patients exhibited typical serum dyslipidemia patterns, with elevated fasting TG followed by delayed and prolonged postprandial response. In IF, significant accumulation of TGs was observed postprandially for both T2D and controls. T2D displayed increased interstitial TGRL and lower HDL in all TPs which became even more pronounced in the later collections (p<0.05). Reduced IF-to-serum ratio of TG was seen at all TPs (p<0.05), VLDL-TG TP3 (p<0.05) and HDL-TG TP1+3 (p<0.05) in T2D.
Conclusions
By exploring the postprandial response in the interstitial compartment, we have identified a dysregulated and protracted metabolism of TGRL in T2D. We are currently exploring whether this is accompanied by an abnormal clearance of these pro-atherogenic particles.
Presenter of 1 Presentation
O018 - Interstitial postprandial hyperlipidemia and remnant lipoproteins in type 2 diabetes mellitus (ID 768)
Abstract
Background and Aims
Diabetic dyslipidemia is characterized by elevated fasting serum TG and VLDL and low HDL-c. Postprandial hypertriglyceridemia and changes in remnant-like lipoprotein particles are well recognized in T2D, but the metabolism and /or clearance of these triglyceride-rich lipoproteins (TGRL) is less clear. The interstitial fluid (IF) represents the immediate environment of most cells in the body and studying postprandial changes in this compartment should provide novel insights into the increased cardiovascular risk observed in T2D.
Methods
Five T2D patients with adequate control of diabetes and matched healthy controls were given a standardized meal (1270 kcal; 70/31g fat/saturated fat; 96/60g carbohydrates/sugar, 64g protein) after fasting overnight. Serum was collected hourly for 9h following meal, and IF was harvested through skin blisters at 3 timepoints (TP1=0-3h, TP2=3-6h, TP3=6-9h). Analyses of serum and IF TG, TGRL and lipoprotein lipids were determined by FPLC and ELISA.
Results
T2D patients exhibited typical serum dyslipidemia patterns, with elevated fasting TG followed by delayed and prolonged postprandial response. In IF, significant accumulation of TGs was observed postprandially for both T2D and controls. T2D displayed increased interstitial TGRL and lower HDL in all TPs which became even more pronounced in the later collections (p<0.05). Reduced IF-to-serum ratio of TG was seen at all TPs (p<0.05), VLDL-TG TP3 (p<0.05) and HDL-TG TP1+3 (p<0.05) in T2D.
Conclusions
By exploring the postprandial response in the interstitial compartment, we have identified a dysregulated and protracted metabolism of TGRL in T2D. We are currently exploring whether this is accompanied by an abnormal clearance of these pro-atherogenic particles.