Giuseppina Caligiuri (France)
INSERM U1148 Laboratory for Vascular Translational ScienceAuthor Of 3 Presentations
Introduction (ID 1623)
Conclusion (ID 1624)
O035 - Phosphorylcholine-targeting vaccination reduces experimental atherosclerosis progression by expanding the B1-like specific B cell pool (ID 726)
Abstract
Background and Aims
Vaccination with phosphorylcholine (PC) reduces experimental atherosclerosis by raising the titer of PC-specific IgM in Apolipoprotein E knockout (ApoEKO) mice.
The epitope of the antibodies elicited by the PC-Vaccine coincides with that of 'natural' anti-pneumococcal antibodies and strikingly similar results can be obtained by vaccination with Prevenar®.
The aim of the present study was therefore to assess whether the atheroprotective effects carried by PC-Vaccine and Prevenar@ are linked to the activation of the germline encoded B cell pool, which is responsible for the production of natural antibodies.
Methods
AID Cre mice were crossed with ApoEKO mice and double transgenic mice were immunized with PC-Vaccine or with Prevenar® as previously reported (DOI:10.1016/j.atherosclerosis.2018.06.903). Three months later, atherosclerotic lesions and natural anti-PC IgM antibodies values were compared to the AID+ (affinity maturated) IgM+ (B1-like) cell pool in the peritoneal cavity and draining lymph nodes.
Results
Discriminant analysis showed that the rise of natural anti-PC IgM antibodies (AB1-2 IgM), the proportion of affinity matured (AID+) B1-like cells (CD19+ CD5+ CD43+ CD1d+ IgM+) having migrated from the draining lymph node to the peritoneal cavity and the lesion size (AUC) were interdependent from each other and that atheroprotection in both PC-Vaccine and Prevenar® mouse groups was proportional to the fraction of affinity matured B1-like cells in the peritoneal cavity and the rise of natural anti-PC IgM antibody production (individual data and bivariate plots are displayed in the figure).
Conclusions
Phosphorylcholine-targeting vaccination boosts affinity maturation of B lymphocytes involved in the production of natural antibodies and prevents experimental atherosclerosis progression.
Presenter of 3 Presentations
Introduction (ID 1623)
Conclusion (ID 1624)
O035 - Phosphorylcholine-targeting vaccination reduces experimental atherosclerosis progression by expanding the B1-like specific B cell pool (ID 726)
Abstract
Background and Aims
Vaccination with phosphorylcholine (PC) reduces experimental atherosclerosis by raising the titer of PC-specific IgM in Apolipoprotein E knockout (ApoEKO) mice.
The epitope of the antibodies elicited by the PC-Vaccine coincides with that of 'natural' anti-pneumococcal antibodies and strikingly similar results can be obtained by vaccination with Prevenar®.
The aim of the present study was therefore to assess whether the atheroprotective effects carried by PC-Vaccine and Prevenar@ are linked to the activation of the germline encoded B cell pool, which is responsible for the production of natural antibodies.
Methods
AID Cre mice were crossed with ApoEKO mice and double transgenic mice were immunized with PC-Vaccine or with Prevenar® as previously reported (DOI:10.1016/j.atherosclerosis.2018.06.903). Three months later, atherosclerotic lesions and natural anti-PC IgM antibodies values were compared to the AID+ (affinity maturated) IgM+ (B1-like) cell pool in the peritoneal cavity and draining lymph nodes.
Results
Discriminant analysis showed that the rise of natural anti-PC IgM antibodies (AB1-2 IgM), the proportion of affinity matured (AID+) B1-like cells (CD19+ CD5+ CD43+ CD1d+ IgM+) having migrated from the draining lymph node to the peritoneal cavity and the lesion size (AUC) were interdependent from each other and that atheroprotection in both PC-Vaccine and Prevenar® mouse groups was proportional to the fraction of affinity matured B1-like cells in the peritoneal cavity and the rise of natural anti-PC IgM antibody production (individual data and bivariate plots are displayed in the figure).
Conclusions
Phosphorylcholine-targeting vaccination boosts affinity maturation of B lymphocytes involved in the production of natural antibodies and prevents experimental atherosclerosis progression.