Gloria Wong (United Kingdom)

University of Reading Food and nutritional sciences
Gloria Wong is a doctoral researcher at the University of Reading. Her main research interest is the impact of dietary fats on platelet function and other cardiovascular risk markers .

Author Of 1 Presentation

O007 - Replacement of dietary SFA with unsaturated fatty acids has favourable effects on platelet function: The Reading, Imperial, Surrey Saturated Fat Cholesterol Intervention (RISSCI)-1 study (ID 137)

Session Type
Lipoproteins and Metabolism
Session Time
11:30 - 13:00
Date
Mon, 31.05.2021
Room
Live Streamed
Lecture Time
12:03 - 12:11

Abstract

Background and Aims

Background: Inappropriate activation of platelet function is known to promote arterial thrombosis leading to myocardial infarction and ischaemic stroke. Dietary fat composition may represent an important modulator of platelet activation, but underlying mechanisms are unclear.

Aim: To determine the effects of replacement of dietary saturated fatty acids (SFA) with unsaturated fatty acids (UFA) on platelet function in healthy men.

Methods

Methods: In this secondary analysis of the RISSCI-1 study (ClinicalTrials.gov Identifier: NCT03270527), platelet function analysis was performed in a subgroup of men (n=51) at the University of Reading. Participants followed two sequential isoenergetic diets for 4 weeks each: high-SFA (18% of total energy (TE)) and low-SFA (≤10%TE). Light transmission platelet aggregometry and flow cytometric measurement of platelet P-selectin exposure, and fibrinogen binding were measured in response to a series of agonists at the end of each intervention diet. Platelet sensitivity to the agonists was established by calculating the EC50.

Results

Results: Platelet sensitivity to a collagen receptor (GPVI) selective agonist was increased after the high compared with low SFA diet (P=0.007)(P-selectin binding, EC50). A significant increase in the extent of platelet aggregation and the capacity to bind fibrinogen in response to ADP were also evident after the high compared with low SFA diet (P≤0.025). Platelet count increased, whereas platelet size decreased in whole blood after the high than low SFA diet (P≤0.01).

Conclusions

Conclusion: Our findings suggest that replacement of dietary SFA with UFA may have beneficial effects on platelet activation by decreasing the expression of P-selectin, fibrinogen binding and reducing platelet aggregation.

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Presenter of 1 Presentation

O007 - Replacement of dietary SFA with unsaturated fatty acids has favourable effects on platelet function: The Reading, Imperial, Surrey Saturated Fat Cholesterol Intervention (RISSCI)-1 study (ID 137)

Session Type
Lipoproteins and Metabolism
Session Time
11:30 - 13:00
Date
Mon, 31.05.2021
Room
Live Streamed
Lecture Time
12:03 - 12:11

Abstract

Background and Aims

Background: Inappropriate activation of platelet function is known to promote arterial thrombosis leading to myocardial infarction and ischaemic stroke. Dietary fat composition may represent an important modulator of platelet activation, but underlying mechanisms are unclear.

Aim: To determine the effects of replacement of dietary saturated fatty acids (SFA) with unsaturated fatty acids (UFA) on platelet function in healthy men.

Methods

Methods: In this secondary analysis of the RISSCI-1 study (ClinicalTrials.gov Identifier: NCT03270527), platelet function analysis was performed in a subgroup of men (n=51) at the University of Reading. Participants followed two sequential isoenergetic diets for 4 weeks each: high-SFA (18% of total energy (TE)) and low-SFA (≤10%TE). Light transmission platelet aggregometry and flow cytometric measurement of platelet P-selectin exposure, and fibrinogen binding were measured in response to a series of agonists at the end of each intervention diet. Platelet sensitivity to the agonists was established by calculating the EC50.

Results

Results: Platelet sensitivity to a collagen receptor (GPVI) selective agonist was increased after the high compared with low SFA diet (P=0.007)(P-selectin binding, EC50). A significant increase in the extent of platelet aggregation and the capacity to bind fibrinogen in response to ADP were also evident after the high compared with low SFA diet (P≤0.025). Platelet count increased, whereas platelet size decreased in whole blood after the high than low SFA diet (P≤0.01).

Conclusions

Conclusion: Our findings suggest that replacement of dietary SFA with UFA may have beneficial effects on platelet activation by decreasing the expression of P-selectin, fibrinogen binding and reducing platelet aggregation.

Hide