148 - DNA methylation of CDKN2B/CDKN2B-AS1 locus enhancer in atherosclerosis (ID 1216)
- Irina A. Goncharova, Russian Federation
- Anton V. Markov, Russian Federation
- Ulia A. Koroleva, Russian Federation
- Nadejda P. Babushkina, Russian Federation
- Ramil R. Salakhov, Russian Federation
- Diana V. Sharysh, Russian Federation
- Alexei A. Sleptcov, Russian Federation
- Nail R. Valiahmetov, Russian Federation
- Maria S. Nazarenko, Russian Federation
- Valery P. Puzyrev, Russian Federation
Abstract
Background and Aims
The 9p21.3 locus, where the long non-coding RNAs (lncRNAs) are located, plays key role in the development of cardiovascular disease, myocardial infarction and atherosclerosis. The aim of our study was to evaluate the DNA methylation of CDKN2B/CDKN2B-AS1 enhancer in atherosclerosis.
Methods
Atherosclerotic plaques (n=22) and normal tissues of carotid arteries (n=18) as well as great saphenous veins (, n=10) and blood leukocytes (n=22) were collected from patients with advanced atherosclerosis (17 males, 5 females, aged 65±7 years). Additionally, we used blood leukocytes (n=14) from healthy individuals (10 males, 4 females, aged 66±8 years). We assessed DNA methylation within enhancer of CDKN2B/CDKN2B-AS1 (31 CpG-sites in chr9:22005065-22005876, GRCh37/hg19) using bisulfite high-throughput sequencing.
Results
The average methylation level for all 31 CpG-sites was [48.83±15.76]% in atherosclerotic plaques; [32.00±13.39]% in normal tissues of carotid arteries; [35.35±9.17]% in saphenous veins; [11.95±10.67]% in blood leukocytes of patients and [11.56±5.65]% in blood leukocytes from healthy individuals.The methylation level in atherosclerotic plaques was higher than in normal tissues of carotid arteries (p<0.01) and blood leukocytes (p<0.5x10-6) for all 31 CpG-sites and higher than in saphenous veins for 26 CpG-sites (p<0.03). Leukocytes of patients with atherosclerosis and healthy donors did not differ in the level of methylation of this locus (p>0.1).
Conclusions
Thus, we observed the tissue-specific DNA methylation within the enhancer of CDKN2B/CDKN2B-AS1 in patients with atherosclerosis. The increase of DNA methylation of this locus in the wall of carotid artery is a risk factor for atherosclerosis.
