Background and Aims: Understanding the molecular mechanisms underlying immune disorder is helpful for improving therapeutic strategies for treating RA. We report the molecular programmes of isolated Treg cells controlling treatment-naïve RA patients using single-cell RNA sequencing (scRNA-seq).
Methods: PBMCs were isolated from early treatment-naïve RA patients. 1×106 cells were cultured with anti-CD3 and anti-CD28in the presence or absence of As2O3 (0.1 µM). Then, Treg cells isolated by FACS from PBMCs . Treg cells were performed by single-cells analysis to gain variance gene.
Results: To define populations and identify genome-wide gene expression, we isolated Treg with or without As2O3 (0.1 µM) from treatment-naïve RA patients and performed scRNA-seq. We performed a volcano plot visualization of gene expression between the cells of early treatment-naive RA patients.
Conclusions: In conclusion, four novel mRNAs were used to generate a valuable target gene, which can serve as an independent indicator to predict the immune status of treatment-naïve RA patients due to their influences on the distinct signaling pathways.