SINGLE-CELL RNA-SEQ ANALYSIS REVEALS THE DIFFERENTIAL GENE EXPRESSION BY ARSENIC TRIOXIDE OF EARLY TREATMENT-NAÏVE RHEUMATOID ARTHRITIS PATIENTS

Session Type
PARALLEL SESSIONS
Date
01.06.2021, Tuesday
Session Time
08:00 - 10:00
Room
HALL B
Lecture Time
09:00 - 09:10
Presenter
  • Chunling Li, China
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Pre Recorded

Abstract

Background and Aims

Background and Aims: Understanding the molecular mechanisms underlying immune disorder is helpful for improving therapeutic strategies for treating RA. We report the molecular programmes of isolated Treg cells controlling treatment-naïve RA patients using single-cell RNA sequencing (scRNA-seq).

Methods

Methods: PBMCs were isolated from early treatment-naïve RA patients. 1×106 cells were cultured with anti-CD3 and anti-CD28in the presence or absence of As2O3 (0.1 µM). Then, Treg cells isolated by FACS from PBMCs . Treg cells were performed by single-cells analysis to gain variance gene.

Results

Results: To define populations and identify genome-wide gene expression, we isolated Treg with or without As2O3 (0.1 µM) from treatment-naïve RA patients and performed scRNA-seq. We performed a volcano plot visualization of gene expression between the cells of early treatment-naive RA patients.

Conclusions

Conclusions: In conclusion, four novel mRNAs were used to generate a valuable target gene, which can serve as an independent indicator to predict the immune status of treatment-naïve RA patients due to their influences on the distinct signaling pathways.

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