Wu Qinan, China

Chongqing University Cancer Hospital and Chongqing Cancer Institute and Chongqing Cancer Hospital Endocrine Nephropathy

Presenter of 3 Presentations

LOWER LIMB ARTERIAL INTERVENTION OR AUTOLOGOUS PLATELET-RICH GEL TREATMENT OF DIABETIC LOWER EXTREMITY ARTERIAL DISEASE WITH FOOT ULCER

Session Name
ADVANCED MEDICAL TECHNOLOGIES TO BE USED IN HOSPITALS
Session Type
E-POSTER VIEWING (EXHIBITION HOURS)
Date
20.02.2020, Thursday
Session Time
09:30 - 15:30
Channel
E-Poster Area
Lecture Time
09:42 - 09:43
Presenter
Authors

Abstract

Background and Aims

To investigate whether the Lower limb vascular intervention or Autologous platelet-rich gel (APR) treatment would benefit patients with diabetic lower extremity arterial disease (LEAD) with foot ulcer.

Methods

A total 82 diabetic LEAD with foot ulcer patients were recruited and divided into 3 groups: group A (30 patients received basal treatment), group B (21 patients received basal and autologous platelet-rich gel treatment), group C (31 patients received basal and Lower limb vascular intervention treatment), all patients had a routine follow-up visit for 6 months. The baseline characters and parameters were detected. After treatment, changes from baseline of all parameters were recorded. The differences among each groups and the relationship among each parameters were conducted.

Results

There were no difference in ABI and major amputation between group A and B (P>0.05), compared with group A and B, the ABI and major amputation rate of the C group were improved (P<0.05); There were no significant difference in Tcpo2, heal rate and minor amputation between A and C group (P>0.05), and compared with group A and C, the Tcpo2, heal rate and minor amputation of B group were improved (P<0.05). The logistic regression analysis indicated that the major amputation was mainly associated with ABI, and the minor amputation was mainly associated with Tcpo2. The lower limb vascular intervention can improve the ABI and reduce the major amputation, and the APR improves the Tcpo2 and reduces the minor amputation.

Conclusions

In diabetic LEAD with foot ulcer, major amputation was mainly associated with ABI, while minor amputation was mainly associated with Tcpo2. Interventional surgery mainly improves the ABI and reduces the incidence of major amputation with the improvement of macro-vascular, and the APR mainly improves the local Tcpo2, and reduces the incidence of minor amputations with the improvement of microcirculation.

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CIRCULATING ANGPTL8 IS ASSOCIATED WITH THE PRESENCE OF METABOLIC SYNDROME AND INSULIN RESISTANCE IN POLYCYSTIC OVARY SYNDROME YOUNG WOMEN

Session Name
NEW TECHNOLOGIES FOR TREATING OBESITY AND PREVENTING RELATED DIABETES
Session Type
E-POSTER VIEWING (EXHIBITION HOURS)
Date
20.02.2020, Thursday
Session Time
09:30 - 15:30
Channel
E-Poster Area
Lecture Time
09:38 - 09:39
Presenter
Authors

Abstract

Background and Aims

ANGPTL8 has been reported to be a regulator of lipid metabolism, and it is associated with insulin resistance (IR) and metabolic syndrome (MetS). We investigated whether ANGPTL8 plays a role in MetS.

Methods

ANGPTL8 and adiponectin concentrations were measured in PCOS patients with or without MetS and in their corresponding healthy controls. The association of circulating ANGPTL8 with adiponectin and other parameters was also examined.

Results

Circulating ANGPTL8 concentrations were higher in PCOS women with MetS than in those without MetS and in the controls (P <0.01). ANGPTL8 was positively correlated with age, BMI, FAT%, WHR, SBP, TG, FBG, HbA1c, Fins, and HOMA-IR (all P <0.01) in the study populations and negatively associated with adiponectin and M-values (P <0.001). In addition, ANGPTL8 was positively correlated with PRL, LH, TEST and FAI and negatively correlated with SHBG (all P <0.01). ROC curve analyses showed that the AUCMetS was 0.87 (P < 0.001), with a sensitivity of 92.4% and specificity of 75.4%, and the AUCIR was 0.82 (P < 0.01), with a sensitivity of 76.4% and specificity of 75.6%.

Conclusions

ANGPTL8 levels progressively decrease from PCOS patients with MetS to those without MetS and may be a serum marker associated with the degree of metabolic disorders.

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EXENATIDE ACTIVATES ADIPONECTIN AND ITS FOLLOWING PATHWAY TO REDUCE THE APOPTOSIS OF THE CARDIOMYOCYTE WHICH INDUCED BY GLUCOLIPOTOXICITY

Session Name
TRIALS IN PROGRESS
Session Type
E-POSTER VIEWING (EXHIBITION HOURS)
Date
20.02.2020, Thursday
Session Time
09:30 - 15:30
Channel
E-Poster Area
Lecture Time
09:44 - 09:45
Presenter
Authors

Abstract

Background and Aims

To explore the mechanism of Exenatide reduces the apoptosis of diabetic neonatal cardiomyocyte

Methods

We used a high glucose/palmitate intervention to mimic Type 2 diabetes in vitro. Neonatal cardiomyocytes were divided into control group(C); diabetes group (D), diabetes treated with Exenatide group(DE); diabetes and block the expression of APPL1 group(DB); diabetes treated with Exenatide and block the expression of APPL1 group (BE). We detected apoptosis rate of cardiomyocytes by TUNEL, the adiponectin levels by ELISA, and the protein expression of APPL1, p-AMPK/T-AMPK, PPARα, and NF-κB by western blotting.

Results

Compared with those in the D group, the level of apoptosis in the C and DE groups was significantly decreased (P<0.05); the concentrations of adiponectin was significantly increased (P<0.05); the expression levels of APPL1, p-AMPK/T-AMPK, PPARα were significantly increased (P<0.05); and the expression levels of NF-κB were decreased (P<0.05). Compared with those in the DB group, the cardiomyocyte apoptosis rate was increased; the expression levels of APPL1, p-AMPK/T-AMPK, PPARα were decreased (P<0.05); the expression levels of NF-κB was significantly increased (P<0.05); and the concentrations of adiponectin was significantly decreased (P<0.05) in BE group.

Conclusions

After diabetic cardiomyocyte were treated with Exenatide, the concentrations of adiponectin was increased, the expression level of the APPL1-AMPK-PPARα axis was increased, and the expression of NF-κB and cardiomyocyte apoptosis rate were decreased, block the expression of APPL1 can reverse this effect.

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