Basic science Poster lunch Poster Display session

17P - Endocrine disrupting chemicals, bisphenol A alters cardiomyocytes beating rate and cell morphology (ID 1835)

Presentation Number
17P
Presentation Topic
Basic science
Lecture Time
13:00 - 13:00
Speakers
  • S. Sheikh Abdul Kadir
Authors
  • S. Sheikh Abdul Kadir
  • Z. Rasidi
  • N. Hanafi
  • R. Kamaludin
  • S. Ab. Rahim
  • R. Siran
  • M. Othman
Session Title
Session Room
Exhibition area, Singapore, Singapore, Singapore
Date
18.11.2017
Session Time
13:00 - 14:00

Abstract

Background

Endocrine disrupting chemicals such as Bisphenol A (BPA) has the potential to cause adverse effects to health by disrupting normal human homeostasis. BPA is widely used plasticizer and it is an ideal polymer for epoxy resins and polycarbonates. Therefore, humans are regularly exposed to this chemical through ingestion of water, food and beverages which are contaminated with BPA. Studies have linked BPA exposure in humans with the risk of cardiovascular disease development, yet the direct effects of BPA on cardiomyocytes morphology have not been entirely explored. In here we aimed to investigate the cytotoxic effects of BPA on cells structure of isolated neonatal rat cardiomyocytes culture.

Methods

Cardiomyocytes were isolated from newborn Sprague Dawley rats. Cardiomyocytes were treated with 10-7 to 10-4 M of BPA and subjected for beating rates experiment, cell viability assay and Scanning Electron microscopy. In beating rate experiment, significant reduction in rates (50% -55%, ± 1.5275, p ≤ 0.05) were observed in cardiomyocytes treated with 10-7 to 10-4 M of BPA.

Results

In beating rate experiment, significant reduction in rates (50% -55%, ± 1.5275, p ≤ 0.05) were observed in cardiomyocytes treated with 10−7 to 10−4 M of BPA. Interestingly, cell viability was markedly reduced (54%, ± 0.0026, p ≤ 0.05) in cardiomyocytes treated with 10−7 M of BPA compared to cells in untreated group and others BPA concentration. Cardiomyocytes show altered cell surface homogeneity after BPA exposure. The signs of flattening cardiomyocytes cell surface, reduction of the size, and blurring of the cell borders were observed and evident after exposure to 10−7 to 10−4 M of BPA.

Conclusions

This study provides in vitro evidence of the potential adverse effects of BPA on cardiomyocytes morphology. However, further investigation would be required to understand how BPA is likely to be potentially hazardous to heart and other heart cells.

Legal entity responsible for the study

Universiti Teknologi MARA Animals Ethics Committee

Funding

None

Disclosure

All authors have declared no conflicts of interest.

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