Yiyi Ma, United States of America
Columbia University NeurologyAuthor Of 2 Presentations
MONOCYTE GENE EXPRESSION SIGNATURES OF HIPPOCAMPAL VOLUME
Abstract
Aims
Hippocampal atrophy is related to Alzheimer's disease, a neurodegenerative disease involved with innate immunity mechanisms through alterations in microglia functions. Using monocyte, a peripheral corresponding cell type to the microglia, we aimed to identify the peripheral transcriptional gene signatures associated with hippocampal volume.
Methods
We included 126 individuals from the Religious Order Study or the Rush Memory and Aging Project who have both measurements of monocyte RNA sequencing and MRI data, and 28 individuals with measurements at two time points. CD14+CD16- monocytes were isolated from the peripheral blood mononuclear cells. Hippocampal volumes were estimated by Freesurfer software. We conducted mixed linear analyses to control the random intra-individual variation and adjusted for age at blood collection, differences in age at blood collection and MRI measurement, sex, total intracranial volume. Those genes with false discovery rate (FDR) P value less than 0.05 were considered as genome-wide significant ones.
Results
There were 8 genes with significantly negative associations with hippocampal volume (BETA<0 and FDR≤0.05), which are TRDMT1, PBLD, RAB29, AHDC1, C18ORF21, HIGD2A, XXYLT1, and PPP1R26. There were 5 genes with significantly positive associations with hippocampal volume (BETA>0 and FDR≤0.05), which are MAPKAPK5, MAPRE1, NHSL1, FAM13A, and NELFB. The pathway enrichment analysis showed that these 13 significant genes were involved in DNA methyltransferase activity, calcium-dependent protein kinase activity, microtubule plus-end binding, tRNA methyltransferase activity, and mitogen-activated protein kinase binding.
Conclusions
We have identified the transcriptomic gene signatures of the hippocampal volume, which needs to be further validated.
LIVE DISCUSSION
- Lena Duchateau, Belgium
- Michael B. Miller, United States of America
- Yiyi Ma, United States of America
- Elisa Navarro, United States of America
- Ellen L. Palmer, United States of America
- Eric Yu, Canada
- Donald R. Miller, United States of America
- Gulnara Akhmadeeva, Russian Federation
- Rudolph E. Tanzi, United States of America
- Gopal Thinakaran, United States of America
Presenter of 2 Presentations
MONOCYTE GENE EXPRESSION SIGNATURES OF HIPPOCAMPAL VOLUME
Abstract
Aims
Hippocampal atrophy is related to Alzheimer's disease, a neurodegenerative disease involved with innate immunity mechanisms through alterations in microglia functions. Using monocyte, a peripheral corresponding cell type to the microglia, we aimed to identify the peripheral transcriptional gene signatures associated with hippocampal volume.
Methods
We included 126 individuals from the Religious Order Study or the Rush Memory and Aging Project who have both measurements of monocyte RNA sequencing and MRI data, and 28 individuals with measurements at two time points. CD14+CD16- monocytes were isolated from the peripheral blood mononuclear cells. Hippocampal volumes were estimated by Freesurfer software. We conducted mixed linear analyses to control the random intra-individual variation and adjusted for age at blood collection, differences in age at blood collection and MRI measurement, sex, total intracranial volume. Those genes with false discovery rate (FDR) P value less than 0.05 were considered as genome-wide significant ones.
Results
There were 8 genes with significantly negative associations with hippocampal volume (BETA<0 and FDR≤0.05), which are TRDMT1, PBLD, RAB29, AHDC1, C18ORF21, HIGD2A, XXYLT1, and PPP1R26. There were 5 genes with significantly positive associations with hippocampal volume (BETA>0 and FDR≤0.05), which are MAPKAPK5, MAPRE1, NHSL1, FAM13A, and NELFB. The pathway enrichment analysis showed that these 13 significant genes were involved in DNA methyltransferase activity, calcium-dependent protein kinase activity, microtubule plus-end binding, tRNA methyltransferase activity, and mitogen-activated protein kinase binding.
Conclusions
We have identified the transcriptomic gene signatures of the hippocampal volume, which needs to be further validated.
LIVE DISCUSSION
- Lena Duchateau, Belgium
- Michael B. Miller, United States of America
- Yiyi Ma, United States of America
- Elisa Navarro, United States of America
- Ellen L. Palmer, United States of America
- Eric Yu, Canada
- Donald R. Miller, United States of America
- Gulnara Akhmadeeva, Russian Federation
- Rudolph E. Tanzi, United States of America
- Gopal Thinakaran, United States of America